Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isocitrate | 10 M, 112% of initial activity | Pyrobaculum calidifontis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
malate | 10 mM, 75% residual activity | Pyrobaculum calidifontis | |
oxaloacetate | 10 mM, 60% residual activity | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | pH 9.5, 50°C | Pyrobaculum calidifontis | |
0.028 | - |
NAD+ | pH 10.5, 50°C | Pyrobaculum calidifontis | |
0.92 | - |
2-oxoglutarate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
5.3 | - |
L-glutamate | pH 10.5, 50°C | Pyrobaculum calidifontis | |
9.8 | - |
NH3 | pH 9.5, 50°C | Pyrobaculum calidifontis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, 109% of initial activity | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
286000 | - |
gel filtration | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | A3MUY9 | - |
- |
Pyrobaculum calidifontis JCM 11548 | A3MUY9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | 2.7% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxobutanoate + NH3 + NADH + H+ | 2.7% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis JCM 11548 | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxocaproate + NH3 + NADH + H+ | 1.3% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-2-aminohexanoate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | - |
Pyrobaculum calidifontis | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | - |
Pyrobaculum calidifontis JCM 11548 | L-glutamate + H2O + NAD+ | - |
r | |
2-oxovalerate + NH3 + NADH + H+ | 6.6% of the activity with 2-oxoglutarate | Pyrobaculum calidifontis | L-valine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 8.4% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 8.4% of the activity with L-glutamate | Pyrobaculum calidifontis JCM 11548 | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Pyrobaculum calidifontis | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | - |
Pyrobaculum calidifontis JCM 11548 | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 3.0% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxo-3-methylvalerate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 1.6% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxo-iso-valerate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 35% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 35% of the activity with L-glutamate | Pyrobaculum calidifontis JCM 11548 | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | 31% of the activity with L-glutamate | Pyrobaculum calidifontis | 2-oxovalerate + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 48200, calculated | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
Pcal_1031 | - |
Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
NAD+ | pH 10.5, 50°C | Pyrobaculum calidifontis | |
17 | - |
L-glutamate | pH 10.5, 50°C | Pyrobaculum calidifontis | |
58 | - |
NADH | pH 9.5, 50°C | Pyrobaculum calidifontis | |
58 | - |
NH3 | pH 9.5, 50°C | Pyrobaculum calidifontis | |
65 | - |
2-oxoglutarate | pH 9.5, 50°C | Pyrobaculum calidifontis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+ | Pyrobaculum calidifontis | |
NAD+ | - |
Pyrobaculum calidifontis | |
NADH | - |
Pyrobaculum calidifontis | |
NADPH | about 1% of th activity with NADH | Pyrobaculum calidifontis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
L-glutamate | pH 10.5, 50°C | Pyrobaculum calidifontis | |
5.9 | - |
NH3 | pH 9.5, 50°C | Pyrobaculum calidifontis | |
71 | - |
2-oxoglutarate | pH 9.5, 50°C | Pyrobaculum calidifontis | |
500 | - |
NAD+ | pH 10.5, 50°C | Pyrobaculum calidifontis | |
5800 | - |
NADH | pH 9.5, 50°C | Pyrobaculum calidifontis |