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Literature summary for 1.4.1.2 extracted from

  • Kawakami, R.; Oyama, M.; Sakuraba, H.; Ohshima, T.
    The unique kinetic behavior of the very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum (2010), Biosci. Biotechnol. Biochem., 74, 884-887.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-aspartate catalytic activator, 2fold activation at 0.1 mM, effect on enzyme kinetics, overview Janthinobacterium lividum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli TOP10 cells Janthinobacterium lividum

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate substrate inhibition Janthinobacterium lividum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the enzyme shows positive cooperativity towards 2-oxoglutarate and NADH, and Michaelis-Menten type kinetics with ammonium chloride in the absence of catalytic activator L-aspartate. L-aspartate effect on enzyme kinetics, overview Janthinobacterium lividum
0.041
-
2-oxoglutarate pH 6.5, 25°C, recombinant enzyme, in presence of 10 mM L-aspartate Janthinobacterium lividum
33.9
-
NH3 as NH4+, pH 6.5, 25°C, recombinant enzyme, in absence of L-aspartate Janthinobacterium lividum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Janthinobacterium lividum
-
2-oxoglutarate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Janthinobacterium lividum A5LH94
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli TOP10 cells by hydrophobic interaction chromatography Janthinobacterium lividum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Janthinobacterium lividum 2-oxoglutarate + NH3 + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
NAD-dependent glutamate dehydrogenase
-
Janthinobacterium lividum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
reductive amination assay at Janthinobacterium lividum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reductive amination assay at Janthinobacterium lividum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Janthinobacterium lividum
NADH
-
Janthinobacterium lividum