Crystallization (Comment) | Organism |
---|---|
co-crystallization of GDH with hexachlorophene and 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one is performed using the hanging drop, vapor-diffusion method at room temperature. In both cases, the drops are formed using a 1:1 mix of protein and reservoir solutions | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one or bithionol, respectively, bind as pairs of stacked compounds at hexameric 2-fold axes between the dimers of subunits | Bos taurus | |
3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one or bithionol, respectively, bind as pairs of stacked compounds at hexameric 2-fold axes between the dimers of subunits | Escherichia coli | |
3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one or bithionol, respectively, bind as pairs of stacked compounds at hexameric 2-fold axes between the dimers of subunits | Tetrahymena | |
bithionol | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one or bithionol, respectively, bind as pairs of stacked compounds at hexameric 2-fold axes between the dimers of subunits | Bos taurus | |
bithionol | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one or bithionol, respectively, bind as pairs of stacked compounds at hexameric 2-fold axes between the dimers of subunits | Escherichia coli | |
bithionol | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one or bithionol, respectively, bind as pairs of stacked compounds at hexameric 2-fold axes between the dimers of subunits | Tetrahymena | |
Hexachlorophene | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that hexachlorophene forms a ring around the internal cavity in GDH through aromatic stacking interactions between the drug and GDH as well as between the drug molecules themselves | Bos taurus | |
Hexachlorophene | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that hexachlorophene forms a ring around the internal cavity in GDH through aromatic stacking interactions between the drug and GDH as well as between the drug molecules themselves | Escherichia coli | |
Hexachlorophene | inhibits GDH in a non-competitive manner with the Vmax being greatly affected without a very large change in Km. Crystal structures discloses that hexachlorophene forms a ring around the internal cavity in GDH through aromatic stacking interactions between the drug and GDH as well as between the drug molecules themselves | Tetrahymena |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
NAD+ | 0.008 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.04, pH 7.5 | Bos taurus | |
0.23 | - |
NAD+ | 0.0015 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.13, pH 7.5 | Bos taurus | |
0.26 | - |
NAD+ | 0.004 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.09, pH 7.5 | Bos taurus | |
0.26 | - |
NAD+ | 0.009 mM inhibitor biothionol, Vmax: 0.05, pH 7.5 | Bos taurus | |
0.31 | - |
NAD+ | without inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.24, pH 7.5 | Bos taurus | |
0.31 | - |
NAD+ | without inhibitor biothionol, Vmax: 0.24, pH 7.5 | Bos taurus | |
0.36 | - |
NAD+ | 0.005 mM inhibitor biothionol, Vmax: 0.13, pH 7.5 | Bos taurus | |
0.39 | - |
L-glutamate | 0.009 mM inhibitor biothionol, Vmax: 0.05, pH 7.5 | Bos taurus | |
0.43 | - |
NAD+ | 0.003 mM inhibitor biothionol, Vmax: 0.22, pH 7.5 | Bos taurus | |
1.06 | - |
L-glutamate | 0.005 mM inhibitor biothionol, Vmax: 0.1, pH 7.5 | Bos taurus | |
1.17 | - |
L-glutamate | 0.003 mM inhibitor biothionol, Vmax: 0.14, pH 7.5 | Bos taurus | |
1.27 | - |
L-glutamate | 0.008 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.05, pH 7.5 | Bos taurus | |
1.38 | - |
L-glutamate | 0.0015 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.13, pH 7.5 | Bos taurus | |
1.38 | - |
L-glutamate | without inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.17, pH 7.5 | Bos taurus | |
1.53 | - |
L-glutamate | 0.004 mM inhibitor 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one, Vmax: 0.09, pH 7.5 | Bos taurus | |
1.62 | - |
L-glutamate | without inhibitor biothionol, Vmax: 0.18, pH 7.5 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00366 | - |
- |
Escherichia coli | - |
- |
- |
Tetrahymena | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NAD+ + H2O | - |
Escherichia coli | 2-oxoglutarate + NADH + NH3 | - |
? | |
L-glutamate + NAD+ + H2O | - |
Bos taurus | 2-oxoglutarate + NADH + NH3 | - |
? | |
L-glutamate + NAD+ + H2O | - |
Tetrahymena | 2-oxoglutarate + NADH + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Escherichia coli |
GDH | - |
Bos taurus |
GDH | - |
Tetrahymena |
glutamate dehydrogenase | - |
Escherichia coli |
glutamate dehydrogenase | - |
Bos taurus |
glutamate dehydrogenase | - |
Tetrahymena |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Bos taurus |
7.5 | - |
assay at | Tetrahymena |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0015 | - |
pH 7.5 | Bos taurus | 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one | |
0.0019 | - |
pH 7.5 | Tetrahymena | Hexachlorophene | |
0.0026 | - |
pH 7.5 | Tetrahymena | 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one | |
0.0039 | - |
pH 7.5 | Bos taurus | Hexachlorophene | |
0.0048 | - |
pH 7.5 | Bos taurus | bithionol | |
0.0059 | - |
pH 7.5 | Tetrahymena | bithionol | |
0.012 | - |
pH 7.5 | Escherichia coli | Hexachlorophene | |
0.017 | - |
pH 7.5 | Escherichia coli | bithionol | |
0.1 | - |
value above 100, pH 7.5 | Escherichia coli | 3-(3,5-dibromo)-4-hydroxybenzylidine-5-iodo-1,3-dihydro-indol-2-one |