Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alkalized extract | from the tuber of Corydalis ternata, activates the hGDH1 up to 3.2fold and hGDH2 up to 4.1fold, hGDH2 is more sensitively affected by 1 mM ADP than hGDH1 on the activation by alkalized extracts | Homo sapiens | |
alkalized extract | from the tuber of Corydalis ternata, increases GDH activity 2.4fold after one year of feeding | Rattus norvegicus | |
protopine | activates the human GDH isozymes, but to a less extent | Homo sapiens | |
protopine | increases GDH activity 1.6fold | Rattus norvegicus |
Application | Comment | Organism |
---|---|---|
medicine | prolonged exposure to Corydalis ternata may be one of the ways to regulate glutamate concentration in brain through the activation of GDH | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of isoenzymes in Escherichia coli as soluble proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y187M | no further stimulation of the mutated GDH isoenzymes by ADP in contrast to the wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | no significant changes in Km values between groups treated with alkalized extract from the tuber of Corydalis ternata or protopine and control groups | Rattus norvegicus | |
0.00111 | - |
2-oxoglutarate | animals treated with alkalized extract from the tuber of Corydalis ternata | Rattus norvegicus | |
0.00131 | - |
2-oxoglutarate | animals treated with protopine | Rattus norvegicus | |
0.00138 | - |
2-oxoglutarate | control group | Rattus norvegicus | |
0.00299 | - |
L-glutamate | animals treated with protopine | Rattus norvegicus | |
0.00302 | - |
L-glutamate | animals treated with alkalized extract from the tuber of Corydalis ternata | Rattus norvegicus | |
0.00321 | - |
L-glutamate | control group | Rattus norvegicus | |
0.00528 | - |
NH4+ | animals treated with alkalized extract from the tuber of Corydalis ternata | Rattus norvegicus | |
0.0054 | - |
NH4+ | animals treated with protopine | Rattus norvegicus | |
0.00613 | - |
NH4+ | control group | Rattus norvegicus | |
0.03609 | - |
NADH | control group | Rattus norvegicus | |
0.03785 | - |
NADH | animals treated with protopine | Rattus norvegicus | |
0.03833 | - |
NADH | animals treated with alkalized extract from the tuber of Corydalis ternata | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
control group | Rattus norvegicus |
0.57 | - |
with protopine | Rattus norvegicus |
0.84 | - |
with alkalized extract from the tuber of Corydalis ternata | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | - |
Rattus norvegicus | L-glutamate + NAD+ + H2O | - |
? | |
2-oxoglutarate + NADPH + NH3 | - |
Homo sapiens | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NH4+ + NADPH | - |
Rattus norvegicus | L-glutamate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Rattus norvegicus |
glutamate dehydrogenase | - |
Homo sapiens |
glutamate dehydrogenase | - |
Rattus norvegicus |
hGDH1 | - |
Homo sapiens |
hGDH2-nerve-specific GDH | - |
Homo sapiens |
house-keeping GDH | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |