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Literature summary for 1.4.1.2 extracted from

  • Lee, K.H.; Huh, J.W.; Choi, M.M.; Yoon, S.Y.; Yang, S.J.; Hong, H.N.; Cho, S.W.
    Regulation of glutamate level in rat brain through activation of glutamate dehydrogenase by Corydalis ternata (2005), Exp. Mol. Med., 37, 371-377.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alkalized extract from the tuber of Corydalis ternata, activates the hGDH1 up to 3.2fold and hGDH2 up to 4.1fold, hGDH2 is more sensitively affected by 1 mM ADP than hGDH1 on the activation by alkalized extracts Homo sapiens
alkalized extract from the tuber of Corydalis ternata, increases GDH activity 2.4fold after one year of feeding Rattus norvegicus
protopine activates the human GDH isozymes, but to a less extent Homo sapiens
protopine increases GDH activity 1.6fold Rattus norvegicus

Application

Application Comment Organism
medicine prolonged exposure to Corydalis ternata may be one of the ways to regulate glutamate concentration in brain through the activation of GDH Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of isoenzymes in Escherichia coli as soluble proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y187M no further stimulation of the mutated GDH isoenzymes by ADP in contrast to the wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information no significant changes in Km values between groups treated with alkalized extract from the tuber of Corydalis ternata or protopine and control groups Rattus norvegicus
0.00111
-
2-oxoglutarate animals treated with alkalized extract from the tuber of Corydalis ternata Rattus norvegicus
0.00131
-
2-oxoglutarate animals treated with protopine Rattus norvegicus
0.00138
-
2-oxoglutarate control group Rattus norvegicus
0.00299
-
L-glutamate animals treated with protopine Rattus norvegicus
0.00302
-
L-glutamate animals treated with alkalized extract from the tuber of Corydalis ternata Rattus norvegicus
0.00321
-
L-glutamate control group Rattus norvegicus
0.00528
-
NH4+ animals treated with alkalized extract from the tuber of Corydalis ternata Rattus norvegicus
0.0054
-
NH4+ animals treated with protopine Rattus norvegicus
0.00613
-
NH4+ control group Rattus norvegicus
0.03609
-
NADH control group Rattus norvegicus
0.03785
-
NADH animals treated with protopine Rattus norvegicus
0.03833
-
NADH animals treated with alkalized extract from the tuber of Corydalis ternata Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
control group Rattus norvegicus
0.57
-
with protopine Rattus norvegicus
0.84
-
with alkalized extract from the tuber of Corydalis ternata Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
Rattus norvegicus L-glutamate + NAD+ + H2O
-
?
2-oxoglutarate + NADPH + NH3
-
Homo sapiens L-glutamate + NADP+ + H2O
-
r
2-oxoglutarate + NH4+ + NADPH
-
Rattus norvegicus L-glutamate + NADP+
-
?

Synonyms

Synonyms Comment Organism
GDH
-
Rattus norvegicus
glutamate dehydrogenase
-
Homo sapiens
glutamate dehydrogenase
-
Rattus norvegicus
hGDH1
-
Homo sapiens
hGDH2-nerve-specific GDH
-
Homo sapiens
house-keeping GDH
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Rattus norvegicus
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens