Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.2 extracted from

  • Jongsareejit, B.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Comparison of two glutamate producing enzymes from the hyperthermophilic archaeon Pyrococcus sp. KOD1 (1998), FEMS Microbiol. Lett., 158, 243-248.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
NADPH
-
Pyrococcus sp.
0.14
-
NADH
-
Pyrococcus sp.
0.2
-
2-oxoglutarate
-
Pyrococcus sp.
0.98
-
NH4+
-
Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
6 * 43000, SDS-PAGE Pyrococcus sp.
284000
-
gel filtration Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression in Escherichia coli Pyrococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
-
Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Pyrococcus sp. L-glutamate + NADP+
-
?
L-glutamate + H2O + NAD+
-
Pyrococcus sp. 2-oxoglutarate + NH3 + NADH + H+
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 43000, SDS-PAGE Pyrococcus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pyrococcus sp.