KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
NADPH | - |
Pyrococcus sp. | |
0.14 | - |
NADH | - |
Pyrococcus sp. | |
0.2 | - |
2-oxoglutarate | - |
Pyrococcus sp. | |
0.98 | - |
NH4+ | - |
Pyrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
6 * 43000, SDS-PAGE | Pyrococcus sp. |
284000 | - |
gel filtration | Pyrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pyrococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Pyrococcus sp. | L-glutamate + NADP+ | - |
? | |
L-glutamate + H2O + NAD+ | - |
Pyrococcus sp. | 2-oxoglutarate + NH3 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 43000, SDS-PAGE | Pyrococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pyrococcus sp. |