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Literature summary for 1.4.1.2 extracted from
- Glutamate dehydroenase of lupin nodules: Purification and properties (1979), Phytochemistry, 18, 1273-1278.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Lupinus luteus |  |
| AMP | - |
Lupinus luteus | |
| citrate | inhibition of amination, no effect on deamination | Lupinus luteus | |
| EDTA | - |
Lupinus luteus | |
| KCN | - |
Lupinus luteus | |
| pyridoxal 5'-phosphate | - |
Lupinus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
6 * 45000, SDS-PAGE | Lupinus luteus |
| 270000 | - |
gel filtration | Lupinus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus luteus | - |
lupin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 4 isoenzymes | Lupinus luteus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| nodule | - |
Lupinus luteus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, up to 1 year, stable | Lupinus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD+ | - |
Lupinus luteus | 2-oxoglutarate + NH3 + NADH + H+ | - |
? | |
| L-norvaline + H2O + NAD+ | deamination at 5% the rate of L-glutamate deamination | Lupinus luteus | 2-oxopentanoate + NH3 + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, SDS-PAGE | Lupinus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
reductive amination | Lupinus luteus |
| 8.8 | - |
oxidative deamination | Lupinus luteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.5 | about 90% of activity maximum at pH 7.9 and 8.5, reductive amination | Lupinus luteus |
| 8.4 | 9.2 | about 90% of activity maximum at pH 8.4 and 9.2, oxidative deamination | Lupinus luteus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Lupinus luteus | |
| NADH | - |
Lupinus luteus | |
| NADPH | can replace NADH only at pH 6, not at pH 7-10 | Lupinus luteus | |
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