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Literature summary for 1.4.1.19 extracted from

  • El Bahr, M.K.; Kutacek, M.; Opatrny, Z.
    L-tryptophan aminotransferase and L-tryptophan dehydrogenase, enzymes of IAA synthesis, in normal and tumorous tobacco tissue cultures (1987), Biochem. Physiol. Pflanz., 182, 213-222.
No PubMed abstract available
Search for more literature for 1.4.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.36
-
 NADH tumor C-58 cell culture Nicotiana tabacum
1.39
-
 NADH tumor T-24 cell culture Nicotiana tabacum
1.46
-
 NADH normal cell culture Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + NAD(P)+ Nicotiana tabacum primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants indol-3-yl-pyruvate + NH3 + NAD(P)H
-
 r

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens
-

Source Tissue

Source Tissue Comment Organism Textmining
tissue culture normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.87
-
 cofactor NADH, activity in normal BV-N strain Nicotiana tabacum
1.22
-
 cofactor NADPH, activity in normal BV-N strain Nicotiana tabacum
1.24
-
 cofactor NADH or NADPH, activity in tumor tobacco tissue strains T-24 Nicotiana tabacum
1.57
-
 cofactor NADH, activity in tumor tobacco tissue strain C-58 Nicotiana tabacum
1.59
-
 cofactor NADPH, activity in tumor tobacco tissue strain C-58 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nicotiana tabacum L-tryptophan + NAD+ + H2O
-
 r
L-tryptophan + NAD(P)+
-
 Nicotiana tabacum indol-3-yl-pyruvate + NH3 + NAD(P)H
-
 r
L-tryptophan + NAD(P)+ primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants Nicotiana tabacum indol-3-yl-pyruvate + NH3 + NAD(P)H
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NAD(P)+
-
 Nicotiana tabacum
NAD(P)H normal Nicotiana tabacum cells: higher specific activity with NADPH than with NADH, tumor cells of Nicotiana tabacum: no difference in specific activity with NADPH and NADH Nicotiana tabacum