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Literature summary for 1.4.1.16 extracted from

  • Liu, W.; Guo, R.; Chen, X.; Li, Z.; Gao, X.; Huang, C.; Wu, Q.; Feng, J.; Zhu, D.
    Structural analysis reveals the substrate-binding mechanism for the expanded substrate specificity of mutant meso-diaminopimelate dehydrogenase (2015), ChemBioChem, 16, 924-929 .
    View publication on PubMed
Search for more literature for 1.4.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium tetani

Crystallization (Commentary)

Crystallization (Comment) Organism
apo form and in complex with meso-diaminopimelate, D-leucine, and 4-methyl-2-oxopentanoic acid, sitting drop vapor diffusion method, using MES (0.1 M, pH 6.0), 15% (w/v) PEG 20000, and 5% (v/v) glycerol Clostridium tetani

Protein Variants

Protein Variants Comment Organism
Q154L/T173I/R199M/P248S/H249N/N276S the mutant shows strongly reduced deamination activity with meso-2,6-diaminoheptanedioate and increased animation activity with pyruvate, phenylpyruvate, and 4-methyl-2-oxopentanoic acid compared to the wild type enzyme Clostridium tetani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+ Clostridium tetani
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NADP+ Clostridium tetani E88
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Clostridium tetani Q890V3
-
-
Clostridium tetani E88 Q890V3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Clostridium tetani

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.022
-
 with 4-methyl-2-oxopentanoic acid as substrate, at pH 9.0 and 30°C Clostridium tetani
0.219
-
 with pyruvate as substrate, at pH 9.0 and 30°C Clostridium tetani
1.036
-
 with meso-2,6-diaminoheptanedioate as substrate, at pH 9.5 and 30°C Clostridium tetani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
-
 Clostridium tetani ?
-
 ?
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
-
 Clostridium tetani E88 ?
-
 ?
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Clostridium tetani L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Clostridium tetani E88 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 r
additional information no activity with phenylpyruvate Clostridium tetani ?
-
 ?
additional information no activity with phenylpyruvate Clostridium tetani E88 ?
-
 ?
pyruvate + NADPH
-
 Clostridium tetani ?
-
 ?
pyruvate + NADPH
-
 Clostridium tetani E88 ?
-
 ?

Synonyms

Synonyms Comment Organism
DAPDH
-
 Clostridium tetani
meso-diaminopimelate dehydrogenase
-
 Clostridium tetani

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Clostridium tetani