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Literature summary for 1.4.1.15 extracted from

  • Misono, H.
    NAD+-dependent lysine dehydrogenase from a plant-pathogenic bacterium, Agrobacterium tumefaciens (1991), Vitamins (Kyoto), 65, 1-12.
No PubMed abstract available
Search for more literature for 1.4.1.15

Activating Compound

Activating Compound Comment Organism Structure
5-Aminovalerate 5 mM, 1.65fold activation Agrobacterium tumefaciens
6-aminocaproate 5 mM, 2.32fold activation Agrobacterium tumefaciens
7-aminoheptanoate 5 mM, 2.3fold activation Agrobacterium tumefaciens
8-aminooctanoate 5 mM, 2.26fold activation Agrobacterium tumefaciens
D-Lysine 5 mM, 1.58fold activation Agrobacterium tumefaciens
D-phenylalanine 5 mM, 1.23fold activation Agrobacterium tumefaciens
D-tryptophan 5 mM, 2.01fold activation Agrobacterium tumefaciens
DL-alpha-hydroxy-n-caproate 5 mM, 1.85fold activation Agrobacterium tumefaciens
DL-Homolysine 5 mM, 1.8fold activation Agrobacterium tumefaciens
L-isoleucine 5 mM, 1.72fold activation Agrobacterium tumefaciens
L-leucine 5 mM, 1.69fold activation Agrobacterium tumefaciens
L-lysine 5 mM, 2.29fold activation Agrobacterium tumefaciens
L-methionine 5 mM, 1.54fold activation Agrobacterium tumefaciens
L-norleucine 5 mM, 2.05fold activation Agrobacterium tumefaciens
L-norvaline 5 mM, 1.71fold activation Agrobacterium tumefaciens
L-phenylalanine 5 mM, 2.09fold activation Agrobacterium tumefaciens
L-phenylglycine 5 mM, 1.74fold activation Agrobacterium tumefaciens
L-Phenyllactate 5 mM, 2.25fold activation Agrobacterium tumefaciens
L-tryptophan 5 mM, 1.24fold activation Agrobacterium tumefaciens
L-tyrosine 5 mM, 1.41fold activation Agrobacterium tumefaciens
L-valine 5 mM, 1.47fold activation Agrobacterium tumefaciens
S-(2-aminoethyl)-L-cysteine 5 mM, 2.32fold activation Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
5,5-dithiobis(2-nitrobenzoate) 0.5 mM, 70% inhibition, 40% inhibition after preincubation with 10 mM L-lysine, 98.5% inhibition after preincubation with 1 mM NAD+ Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NAD+
-
 Agrobacterium tumefaciens
1.85
-
 deamino-NAD+
-
 Agrobacterium tumefaciens
3.13
-
 3-acetylpyridine-NAD+
-
 Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Agrobacterium tumefaciens

Reaction

Reaction Comment Organism Reaction ID
L-lysine + NAD+ = 1,2-didehydropiperidine-2-carboxylate + NH3 + NADH + H+ the pro-R hydrogen at the prochiral C-6 carbon of L-lysine is specifically abstracted and transferred to the pro-R position at C-4 of NAD+ Agrobacterium tumefaciens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 3-acetylpyridine-NAD+
-
 Agrobacterium tumefaciens L-glutamine + 2-oxoglutarate + 3-acetylpyridine-NADH + H+
-
 r
L-glutamate + deamino-NAD+
-
 Agrobacterium tumefaciens L-glutamine + 2-oxoglutarate + deamino-NADH + H+
-
 r
L-lysine + NAD+ + H2O
-
 Agrobacterium tumefaciens 1,2-didehydropiperidine-6-carboxylate + NH3 + NADH
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 in the absence of lysine, approx. 50% activity after 10 min at 40°C Agrobacterium tumefaciens
30 60 in the presence of 5 mM L-lysine, approx. 50% activity after 10 min at 55°C Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ can not replace NAD+ Agrobacterium tumefaciens