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Literature summary for 1.4.1.14 extracted from

  • Roon, R.J.; Even, H.I.; Larimore, F.
    Glutamate synthase: Properties of the reduced nicotinamide adenine dinucleotide-dependent enzyme from Saccharomyces cerevisiae (1974), J. Bacteriol., 118, 89-95.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.14

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Saccharomyces cerevisiae
25% glycerol stabilizes Saccharomyces cerevisiae
EDTA stabilizes Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by the 20 common amino acids at a concentration of 20 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 NADH
-
 Saccharomyces cerevisiae
0.14
-
 2-oxoglutarate
-
 Saccharomyces cerevisiae
1
-
 L-glutamine
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Saccharomyces cerevisiae glutamate biosynthesis glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH Saccharomyces cerevisiae X2180A glutamate biosynthesis glutamate + NAD+
-
 ir

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae X2180A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
20fold partial purification Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
25°C, 4°C or -20°C, relatively unstable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Saccharomyces cerevisiae L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH specific for NADH Saccharomyces cerevisiae L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Saccharomyces cerevisiae X2180A L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH specific for NADH Saccharomyces cerevisiae X2180A L-glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH glutamate biosynthesis Saccharomyces cerevisiae glutamate + NAD+
-
 ir
L-glutamine + 2-oxoglutarate + NADH glutamate biosynthesis Saccharomyces cerevisiae X2180A glutamate + NAD+
-
 ir
additional information no activity with L-asparagine, D-glutamine, pyruvate, oxaloacetate or NADPH Saccharomyces cerevisiae ?
-
 ?
additional information no activity with L-asparagine, D-glutamine, pyruvate, oxaloacetate or NADPH Saccharomyces cerevisiae X2180A ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1 7.7
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 9 at pH 6 and 9 about 50% of activity maximum Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Saccharomyces cerevisiae
NADH specific for NADH as reductant Saccharomyces cerevisiae