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Literature summary for 1.4.1.14 extracted from
- Two isoenzymes of NADH-dependent glutamate synthase in root nodules of Phaseolus vulgaris L.. Purification, properties and activity changes during nodule development (1988), Plant Physiol., 88, 1411-1417.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Phaseolus vulgaris |
| dithiothreitol stabilizes | Phaseolus vulgaris |
| EDTA stabilizes | Phaseolus vulgaris |
| highly labile enzyme activity | Phaseolus vulgaris |
| phenylmethylsulphonyl fluoride is used as precaution against proteolysis | Phaseolus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azaserine | completely inhibited by 0.4 mM | Phaseolus vulgaris |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0052 | - |
NADH | NADH-GOGAT II | Phaseolus vulgaris | |
| 0.014 | - |
NADH | NADH-GOGAT I | Phaseolus vulgaris | |
| 0.022 | - |
2-oxoglutarate | NADH-GOGAT I | Phaseolus vulgaris | |
| 0.087 | - |
2-oxoglutarate | NADH-GOGAT II | Phaseolus vulgaris | |
| 0.24 | - |
L-glutamine | NADH-GOGAT II | Phaseolus vulgaris | |
| 0.77 | - |
L-glutamine | NADH-GOGAT I | Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
NADH-GOGAT II, gel filtration | Phaseolus vulgaris |
| 240000 | - |
NADH-GOGAT I, gel filtration | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH | Phaseolus vulgaris | ammonia assimilation | glutamate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
2 isoenzymes: NADH-GOGAT I and NADH-GOGAT II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes: NADH-GOGAT I with 877fold purification and NADH-GOGAT II with 631fold purification | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root nodule | NADH-GOGAT II is twice as active as NADH-GOGAT I in mature nodules | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
NADH-GOGAT I | Phaseolus vulgaris |
| 8.05 | - |
NADH-GOGAT II | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Phaseolus vulgaris | L-glutamate + NAD+ | - |
? | |
| L-glutamine + 2-oxoglutarate + NADH | ammonia assimilation | Phaseolus vulgaris | glutamate + NAD+ | - |
? | |
| additional information | no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH | Phaseolus vulgaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 200000, SDS-PAGE | Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
NADH-GOGAT I/II | Phaseolus vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
very sensitive to low temperatures | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
NADH-GOGAT I, broad pH-optimum centering at pH 8 | Phaseolus vulgaris |
| 8.5 | - |
NADH-GOGAT II, narrow pH-optimum of 8.5 | Phaseolus vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | specific for NADH as reductant | Phaseolus vulgaris | |
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