General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Phaseolus vulgaris |
dithiothreitol stabilizes | Phaseolus vulgaris |
EDTA stabilizes | Phaseolus vulgaris |
highly labile enzyme activity | Phaseolus vulgaris |
phenylmethylsulphonyl fluoride is used as precaution against proteolysis | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azaserine | completely inhibited by 0.4 mM | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
NADH | NADH-GOGAT II | Phaseolus vulgaris | |
0.014 | - |
NADH | NADH-GOGAT I | Phaseolus vulgaris | |
0.022 | - |
2-oxoglutarate | NADH-GOGAT I | Phaseolus vulgaris | |
0.087 | - |
2-oxoglutarate | NADH-GOGAT II | Phaseolus vulgaris | |
0.24 | - |
L-glutamine | NADH-GOGAT II | Phaseolus vulgaris | |
0.77 | - |
L-glutamine | NADH-GOGAT I | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
NADH-GOGAT II, gel filtration | Phaseolus vulgaris |
240000 | - |
NADH-GOGAT I, gel filtration | Phaseolus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | Phaseolus vulgaris | ammonia assimilation | glutamate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
2 isoenzymes: NADH-GOGAT I and NADH-GOGAT II | - |
Purification (Comment) | Organism |
---|---|
2 isoenzymes: NADH-GOGAT I with 877fold purification and NADH-GOGAT II with 631fold purification | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | NADH-GOGAT II is twice as active as NADH-GOGAT I in mature nodules | Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.8 | - |
NADH-GOGAT I | Phaseolus vulgaris |
8.05 | - |
NADH-GOGAT II | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Phaseolus vulgaris | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | ammonia assimilation | Phaseolus vulgaris | glutamate + NAD+ | - |
? | |
additional information | no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH | Phaseolus vulgaris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 200000, SDS-PAGE | Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
NADH-GOGAT I/II | Phaseolus vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
very sensitive to low temperatures | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
NADH-GOGAT I, broad pH-optimum centering at pH 8 | Phaseolus vulgaris |
8.5 | - |
NADH-GOGAT II, narrow pH-optimum of 8.5 | Phaseolus vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | specific for NADH as reductant | Phaseolus vulgaris |