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Literature summary for 1.4.1.14 extracted from
- Purification and characterization of NADH-glutamate synthase from alfalfa root nodules (1989), Plant Physiol., 90, 351-358.
General Stability
| General Stability | Organism |
|---|---|
| highly labile enzyme activity | Medicago sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azaserine | glutamine antagonist, completely inhibited by 10 mM | Medicago sativa |  |
| citrate | inhibited by high concentrations, 40 mM | Medicago sativa | |
| L-glutamate | - |
Medicago sativa | |
| malate | inhibited by high concentrations, 40 mM | Medicago sativa | |
| additional information | not inhibited by 2,2-dipyridyl or N-ethylmaleimide | Medicago sativa | |
| NAD+ | - |
Medicago sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0042 | - |
NADH | - |
Medicago sativa | |
| 0.033 | - |
2-oxoglutarate | - |
Medicago sativa | |
| 0.466 | - |
L-glutamine | - |
Medicago sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
1 * 200000, SDS-PAGE | Medicago sativa |
| 235000 | - |
gel filtration | Medicago sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH | Medicago sativa | key enzyme in pathway of assimilation of symbiotically fixed N2 into amino acids | glutamate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 208fold purification | Medicago sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root nodule | high enzyme activity in nodules compared to roots and other organs | Medicago sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.1 | - |
- |
Medicago sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 50% glycerol, 100 mM potassium phosphate, 3 mM DTT, stable for up to 1 month | Medicago sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH | - |
Medicago sativa | L-glutamate + NAD+ | - |
? | |
| L-glutamine + 2-oxoglutarate + NADH | key enzyme in pathway of assimilation of symbiotically fixed N2 into amino acids | Medicago sativa | glutamate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 200000, SDS-PAGE | Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Medicago sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | below and above activity declines rapidly | Medicago sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Medicago sativa | |
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Release 2023.1 (January 2023)
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