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Literature summary for 1.4.1.14 extracted from
- Glutamate synthase from Escherichia coli, Klebsiella aerogenes, and Saccharomyces cerevisiae (1985), Methods Enzymol., 113, 327-337.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (2S)-2-amino-4-(S-methylsulfonimidoyl)butanoic acid | - |
Saccharomyces cerevisiae |  |
| D-glutamine | - |
Saccharomyces cerevisiae | |
| DL-ethionine sulfone | - |
Saccharomyces cerevisiae | |
| DL-homocysteic acid | - |
Saccharomyces cerevisiae | |
| L-Albizziin | - |
Saccharomyces cerevisiae | |
| L-homocysteine sulfonamide | very potent transition state inhibitor, competitive to L-glutamine | Saccharomyces cerevisiae | |
| L-methionine sulfone | markedly inhibits | Saccharomyces cerevisiae | |
| L-methionine-SR-sulfoxide | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
NADH | - |
Saccharomyces cerevisiae | |
| 0.04 | - |
2-oxoglutarate | - |
Saccharomyces cerevisiae | |
| 0.28 | - |
L-glutamine | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
| 169000 | - |
1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
| 265000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.7 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + NADH | low activity when NH3 is substituted for glutamine, NH4Cl: 2.7% of the activity with L-glutamine | Saccharomyces cerevisiae | L-glutamate + NAD+ | - |
? | |
| L-glutamine + 2-oxoglutarate + NADH | specific for NADH | Saccharomyces cerevisiae | L-glutamate + NAD+ | - |
? | |
| additional information | no activity with 2-oxovalerate, 2-oxopimelate, 2-oxoadipate, oxaloacetate, D-glutamine, L- or D-asparagine, L- or D-homoglutamine, alpha-methyl-L-glutamine, DL-beta-glutamine, L-isoglutamine, glutaramide or glutaramate | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 169000 + 1 * 61000, SDS-PAGE in presence of 1% 2-mercaptoethanol | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | glutamine-dependent NADH oxidase activity | Saccharomyces cerevisiae |
| 9 | - |
ammonia-dependent NADH oxidase activity, rate steadily increases from pH 6 to 9 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADPH | Saccharomyces cerevisiae | |
| NADH | specific for NADH as reductant | Saccharomyces cerevisiae | |
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