Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | 35% inhibition at 0.0025 mM, when reaction is initiated with 0.5 mM L-glutamine, 73% inhibition at 0.0025 mM, when reaction is initiated with 2.5 mM 2-oxoglutarate | Rhodospirillum rubrum | |
azaserine | 38% inhibition at 0.0125 mM when reaction is initiated with 0.5 mM L-glutamine and 69% inhibition at 0.0125 mM when reaction is initiated with 2.5 mM 2-oxoglutarate | Rhodospirillum rubrum | |
L-aspartate | - |
Rhodospirillum rubrum | |
L-methionine | - |
Rhodospirillum rubrum | |
NADP+ | competitive inhibitor with NADPH, 54% inhibition at 1 mM | Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2-oxoglutarate | - |
Rhodospirillum rubrum | |
0.016 | - |
NADPH | - |
Rhodospirillum rubrum | |
0.065 | - |
L-glutamine | - |
Rhodospirillum rubrum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein | Rhodospirillum rubrum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 152000 + x * 53000, SDS-PAGE | Rhodospirillum rubrum |
152000 | - |
x * 152000 + x * 53000, SDS-PAGE | Rhodospirillum rubrum |
800000 | - |
gel filtration, presence of Cl- | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH | Rhodospirillum rubrum | main route for assimilation of ammonium compounds | L-glutamate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate precipitation and column chromatography on DEAE-Sepharose, 2',5'-ADP-Sepharose and Sephacryl S-300 | Rhodospirillum rubrum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
- |
Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH | main route for assimilation of ammonium compounds | Rhodospirillum rubrum | L-glutamate + NADP+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADPH + H+ | no activity with NH4+ | Rhodospirillum rubrum | L-glutamate + NADP+ | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | the activity with 10 mM NH4+ ions is less than 2% that with L-glutamine | Rhodospirillum rubrum | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 152000 + x * 53000, SDS-PAGE | Rhodospirillum rubrum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoenzyme | Rhodospirillum rubrum | |
NADH | no activity with NADH | Rhodospirillum rubrum | |
NADPH | - |
Rhodospirillum rubrum |