Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 27% inhibition at 50 mM | Bacillus licheniformis | |
DL-methionine sulfoxide | potent inhibitor | Bacillus licheniformis | |
glycine | 5% inhibition at 5 mM | Bacillus licheniformis | |
L-alanine | 5% inhibition at 5 mM | Bacillus licheniformis | |
L-histidine | 5% inhibition at 5 mM | Bacillus licheniformis | |
L-leucine | 16% inhibition at 5 mM | Bacillus licheniformis | |
L-methionine | 16% inhibition at 5 mM | Bacillus licheniformis | |
L-methionine DL-sulfoximine | potent inhibitor | Bacillus licheniformis | |
L-methionine sulfone | potent inhibitor | Bacillus licheniformis | |
L-serine | 10% inhibition at 5 mM | Bacillus licheniformis | |
MgCl2 | 31% inhibition at 50 mM | Bacillus licheniformis | |
MnCl2 | 64% inhibition at 50 mM | Bacillus licheniformis | |
additional information | relatively insensitive to inhibition by amino acids, keto acids or various nucleotides, 38% inhibition with a combination of each 5 mM L-methionine, L-leucine, L-serine, L-histidine, L-glycine and L-alanine | Bacillus licheniformis | |
NADP+ | competitive inhibition with respect to NADPH; potent inhibitor | Bacillus licheniformis | |
NADPH | - |
Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADPH | - |
Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein | Bacillus licheniformis | |
Iron | 8.1 iron atoms per MW 220000 | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 158000 + 1 * 54000, SDS-PAGE | Bacillus licheniformis |
158000 | - |
1 * 158000 + 1 * 54000, SDS-PAGE | Bacillus licheniformis |
220000 | - |
sucrose density gradient sedimentation | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on DEAE-Sephacel, Bio-Gel A 1.5 m, Red Sepharose CL6B and Sephadex G-25 | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.9 | - |
- |
Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | highly specific | Bacillus licheniformis | L-glutamate + NADP+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Bacillus licheniformis | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 158000 + 1 * 54000, SDS-PAGE | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
glutamine-dependent activity | Bacillus licheniformis |
8.7 | - |
ammonia-dependent activity | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | ratio FAD: FMN is 1.2 | Bacillus licheniformis | |
flavin | 2 flavin moieties per 220000 MW | Bacillus licheniformis | |
additional information | the enzyme contains 8.1 acid-labile sulfur atoms per 220000-dalton dimer | Bacillus licheniformis | |
NADPH | - |
Bacillus licheniformis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADP | competitive inhibition with NADPH | Bacillus licheniformis |