Cloned (Comment) | Organism |
---|---|
gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of ald knockout strain RVW7 from wild-type strain H37Rv | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of ald and glyoxylate dehydrogenae activities, overview | Mycobacterium tuberculosis | |
0.0384 | - |
NADH | pH 6.4, 22°C, recombinant His6-tagged enzyme | Mycobacterium tuberculosis | |
0.1328 | - |
NAD+ | pH 6.4, 22°C, recombinant His6-tagged enzyme | Mycobacterium tuberculosis | |
2.3 | - |
NH3 | pH 6.4, 22°C, recombinant His6-tagged enzyme | Mycobacterium tuberculosis | |
2.8 | - |
pyruvate | pH 6.4, 22°C, recombinant His6-tagged enzyme | Mycobacterium tuberculosis | |
4.3 | - |
L-alanine | pH 6.4, 22°C, recombinant His6-tagged enzyme | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | minor fraction | Mycobacterium tuberculosis | 5829 | - |
membrane | major fraction | Mycobacterium tuberculosis | 16020 | - |
additional information | no enzyme detected in the extracellular medium | Mycobacterium tuberculosis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | Mycobacterium tuberculosis | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | Mycobacterium tuberculosis H37Rv | - |
pyruvate + NH3 + NADH + H+ | - |
r | |
additional information | Mycobacterium tuberculosis | enzyme Ald has both pyruvate and glyoxylate aminating activities | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | enzyme Ald has both pyruvate and glyoxylate aminating activities | ? | - |
? | |
pyruvate + NH3 + NADH + H+ | Mycobacterium tuberculosis | - |
L-alanine + H2O + NAD+ | - |
r | |
pyruvate + NH3 + NADH + H+ | Mycobacterium tuberculosis H37Rv | - |
L-alanine + H2O + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene ald | - |
Mycobacterium tuberculosis H37Rv | - |
gene ald | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities in different strains, overview | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + NAD+ | - |
Mycobacterium tuberculosis | pyruvate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | - |
Mycobacterium tuberculosis H37Rv | pyruvate + NH3 + NADH + H+ | - |
r | |
additional information | enzyme Ald has both pyruvate and glyoxylate aminating activities | Mycobacterium tuberculosis | ? | - |
? | |
additional information | enzyme Ald has both pyruvate and glyoxylate aminating activities | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
pyruvate + NH3 + NADH + H+ | - |
Mycobacterium tuberculosis | L-alanine + H2O + NAD+ | - |
r | |
pyruvate + NH3 + NADH + H+ | - |
Mycobacterium tuberculosis H37Rv | L-alanine + H2O + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ALD | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
ALD activity | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycobacterium tuberculosis | |
NADH | - |
Mycobacterium tuberculosis |
Organism | Comment | Expression |
---|---|---|
Mycobacterium tuberculosis | transcription of ald is induced when alanine is the sole nitrogen source | up |
General Information | Comment | Organism |
---|---|---|
malfunction | an ald knockout strain grows without alanine or glycine and is able to utilize glycine but not alanine as a nitrogen source | Mycobacterium tuberculosis |
physiological function | enzyme Ald may have several functions, including ammonium incorporation and alanine breakdown. Ald plays an essential role in the utilization of alanine but not of glycine. Ald is not essential for the breakdown of glycine | Mycobacterium tuberculosis |