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Literature summary for 1.4.1.1 extracted from

  • Lu, X.; Yi, Q.; Zhang, G.; Zhu, X.; Zhou, H.; Dong, H.
    Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002 (2013), Acta Crystallogr. Sect. F, 69, 934-936.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ald, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Priestia megaterium

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, mixed with 0.1 M HEPES, pH 8.0, 12% w/v PEG 8000, and 8% v/v ethylene glycol, 4°C, method optimization, X-ray diffraction structure determination and analysis at 2.35 A resolution Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, recombinant His6-tagged enzme, SDS-PAGE Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + H2O + NAD+ Priestia megaterium
-
pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ Priestia megaterium WSH-002
-
pyruvate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Priestia megaterium A0A0L1M9S8 gene ald
-
Priestia megaterium WSH-002 A0A0L1M9S8 gene ald
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + H2O + NAD+
-
Priestia megaterium pyruvate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+
-
Priestia megaterium WSH-002 pyruvate + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 42000, recombinant His6-tagged enzme, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
L-AlaDH
-
Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Priestia megaterium
NADH
-
Priestia megaterium