Application | Comment | Organism |
---|---|---|
nutrition | Lactococcus lactis strain NZ9000 expressing the enzyme from Bacillus subtilis can be used in imporvement of dairy fermentation for developing healthy yogurts with sweet taste or other fermented dairy foods | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
gene ald, functional heterologous expression in Escherichia coli strain JM109, as GST-tagged protein, and in Lactococcus lactis strain NZ9000 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, recombinant GST-tagged enzyme, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NH3 + NADH + H+ | Bacillus subtilis | - |
L-alanine + H2O + NAD+ | - |
? | |
pyruvate + NH3 + NADH + H+ | Bacillus subtilis natto | - |
L-alanine + H2O + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene ald | - |
Bacillus subtilis natto | - |
gene ald | - |
no activity in Lactococcus lactis | - |
strain NZ9000 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chrmatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NH3 + NADH + H+ | - |
Bacillus subtilis | L-alanine + H2O + NAD+ | - |
? | |
pyruvate + NH3 + NADH + H+ | - |
Bacillus subtilis natto | L-alanine + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, recombinant GST-tagged enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
ALADH | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 42 | assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Bacillus subtilis |