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Literature summary for 1.4.1.1 extracted from
- Alanine production in an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase (2007), Appl. Microbiol. Biotechnol., 76, 819-825.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain TBLA-1, an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli derived from the pyruvate producing K-12 strain W1485lip2 | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0109 | - |
recombinant enzyme, in 750 mM NH4OH/NH4Cl buffer (pH 9.0) with 30 mM sodium pyruvate | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala + H2O + NAD+ | - |
Geobacillus stearothermophilus | pyruvate + NH3 + NADH | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH | - |
Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Geobacillus stearothermophilus | |
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Release 2023.1 (January 2023)
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