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Literature summary for 1.4.1.1 extracted from

  • Schröder, I.; Vadas, A.; Johnson, E.; Lim, S.; Monbouquette, H.G.
    A novel archaeal alanine dehydrogenase homologous to ornithine cyclodeaminase and mu-crystallin (2004), J. Bacteriol., 186, 7680-7689.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

General Stability

General Stability Organism
optimal KCl concentration for stability is 1.5 M Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
Ketobutyrate
-
 Archaeoglobus fulgidus
L-2-aminobutyrate
-
 Archaeoglobus fulgidus
L-Ala
-
 Archaeoglobus fulgidus
NADH
-
 Archaeoglobus fulgidus
pyruvate
-
 Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADH pH 8.5, 82°C Archaeoglobus fulgidus
0.085
-
 L-2-aminobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
0.16
-
 pyruvate pH 8.5, 82°C Archaeoglobus fulgidus
0.48
-
 2-oxobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
0.6
-
 NAD+ pH 8.5, 82°C Archaeoglobus fulgidus
0.71
-
 L-Ala pH 8.5, 82°C Archaeoglobus fulgidus
0.97
-
 oxaloacetate pH 8.5, 82°C Archaeoglobus fulgidus
17.3
-
 NH4+ pH 8.5, 82°C Archaeoglobus fulgidus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Archaeoglobus fulgidus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Archaeoglobus fulgidus
76000
-
 gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
203
-
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketocaproate + NH3 + NADH
-
 Archaeoglobus fulgidus 2-aminocaproate + H2O + NAD+
-
 ?
3-fluoropyruvate + NH3 + NADH
-
 Archaeoglobus fulgidus ? + H2O + NAD+
-
 ?
ketovalerate + NH3 + NADH
-
 Archaeoglobus fulgidus 2-aminovalerate + H2O + NAD+
-
 ?
L-2-aminobutyrate + H2O + NAD+
-
 Archaeoglobus fulgidus 2-oxobutyrate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Archaeoglobus fulgidus pyruvate + NH3 + NADH
-
 r
L-Asp + H2O + NAD+
-
 Archaeoglobus fulgidus oxaloacetate + NH3 + NADH
-
 r
L-Ile + H2O + NAD+
-
 Archaeoglobus fulgidus 2-oxo-3-methylpentanoate + NH3 + NADH
-
 ?
L-Ser + H2O + NAD+
-
 Archaeoglobus fulgidus 3-hydroxypyruvate + NH3 + NADH
-
 r
L-Thr + H2O + NAD+
-
 Archaeoglobus fulgidus 3-hydroxy-2-oxobutyrate + NH3 + NADH
-
 ?
L-Val + H2O + NAD+
-
 Archaeoglobus fulgidus 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
phenylpyruvate + NH3 + NADH
-
 Archaeoglobus fulgidus L-phenylalanine + H2O + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
ALADH
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
82
-
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 in presence of 1.5 M KCl stable for 3 months Archaeoglobus fulgidus
60
-
 less than 5% loss of activity after 65 h Archaeoglobus fulgidus
90
-
 half-life: 55 h in presence of 1.5 M Kcl Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1
-
 NAD+ pH 8.5, 82°C Archaeoglobus fulgidus
6.1
-
 L-Ala pH 8.5, 82°C Archaeoglobus fulgidus
9.6
-
 L-2-aminobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
113
-
 oxaloacetate pH 8.5, 82°C Archaeoglobus fulgidus
118
-
 NADH pH 8.5, 82°C Archaeoglobus fulgidus
118
-
 pyruvate pH 8.5, 82°C Archaeoglobus fulgidus
118
-
 NH4+ pH 8.5, 82°C Archaeoglobus fulgidus
143
-
 2-oxobutyrate pH 8.5, 82°C Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 7.4 oxidative deamination Archaeoglobus fulgidus
7.5
-
 reductive amination Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
6.1 8.3 pH 6.1: about 60% of maximal activity, pH 8.3: about 60% of maximal activity, reductive amination Archaeoglobus fulgidus
6.2 8 pH 6.2: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, oxidative deamination Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ does not serve as coenzyme Archaeoglobus fulgidus
NADH NADPH does not serve as coenzyme Archaeoglobus fulgidus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
 NADH pH 8.5, 82°C Archaeoglobus fulgidus
4.7
-
 L-2-aminobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
4.8
-
 2-Ketobutyrate pH 8.5, 82°C Archaeoglobus fulgidus
16.8
-
 L-Ala pH 8.5, 82°C Archaeoglobus fulgidus
27.8
-
 pyruvate pH 8.5, 82°C Archaeoglobus fulgidus

pI Value

Organism Comment pI Value Maximum pI Value
Archaeoglobus fulgidus calculation from nucleotide sequence
-
 4.9