Literature summary for 1.4.1.1 extracted from

Irwin, J.A.; Gudmundsson, H.M.; Marteinsson, V.T.; Hreggvidsson, G.O.; Lanzetti, A.J.; Alfredsson, G.A.; Engel, P.C.
Characterization of alanine and malate dehydrogenases from a marine psychrophile strain PA-43 (2001), Extremophiles, 5, 199-211.

Search for more literature for 1.4.1.1

General Stability

General Stability	Organism
more than 75% loss of activity after two freeze-thaw cycles and storage at -20°C for 1 week, stabilized by addition of 50% glycerol	Bacteria

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42300	-	6 * 42300, SDS-PAGE	Bacteria
260000	-	gel filtration	Bacteria

Organism

Organism	UniProt	Comment	Textmining
Bacteria	-	psychrophile strain PA-43, isolated from a sea urchin	-
Phormidium lapideum	-	-	-
Shewanella sp.	-	-	-
Vibrio proteolyticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bacteria

Storage Stability

Storage Stability	Organism
-20°C, in presence of 50% glycerol, stable for up to 5 months	Bacteria
4°C or -20°C, less than 0.2 mg/ml, enzyme loses most of its activity in less than 2 weeks	Bacteria
4°C, pH 6-8, 50 mM phosphate buffer, stable for at least 3 days	Bacteria

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala + H2O + NAD+	-	Bacteria	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Vibrio proteolyticus	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Shewanella sp.	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Phormidium lapideum	pyruvate + NH3 + NADH	-	r

Subunits

Subunits	Comment	Organism
hexamer	-	Shewanella sp.
hexamer	-	Phormidium lapideum
hexamer	6 * 42300, SDS-PAGE	Bacteria

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	50	NAD+ reduction	Bacteria

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	65	40°C: 70% of maximal activity, 65°C: about 50% of maximal activity	Bacteria

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	90 min, stable	Bacteria
42	-	90 min, stable	Bacteria
50	-	-	Phormidium lapideum
50	-	half-life: 42 min	Bacteria
56	-	half-life: 2.25 min	Bacteria
60	-	-	Phormidium lapideum
60	-	half-life: 15 s	Bacteria

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	NADH oxidation	Bacteria
9	-	reductive amination of pyruvate	Bacteria
10	-	NAD+ reduction	Bacteria
10.5	-	oxidative deamination of L-Ala	Bacteria

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Bacteria
NAD+	-	Vibrio proteolyticus
NAD+	-	Shewanella sp.
NAD+	-	Phormidium lapideum
NADH	-	Bacteria
NADH	-	Vibrio proteolyticus
NADH	-	Shewanella sp.
NADH	-	Phormidium lapideum

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Release 2023.1 (January 2023)