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Literature summary for 1.4.1.1 extracted from

  • Sawa, Y.; Tani, M.; Murata, K.; Shibata, H.; Ochiai, H.
    Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidium lapideum (1994), J. Biochem., 116, 995-1000.
    View publication on PubMed
Search for more literature for 1.4.1.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Phormidium lapideum

General Stability

General Stability Organism
partial inactivation by dilution Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Phormidium lapideum
Cibacron blue
-
 Phormidium lapideum
Cu2+
-
 Phormidium lapideum
Hg2+
-
 Phormidium lapideum
L-Ala
-
 Phormidium lapideum
L-Phe reductive amination of pyruvate, 10 mM, 28% inhibition Phormidium lapideum
L-Ser reductive amination of pyruvate Phormidium lapideum
L-Thr reductive amination of pyruvate, 10 mM, 37% inhibition Phormidium lapideum
L-Trp reductive amination of pyruvate, 10 mM, 29% inhibition Phormidium lapideum
PCMB
-
 Bacillus subtilis
PCMB
-
 Phormidium lapideum
pyridoxal 5'-phosphate
-
 Phormidium lapideum
Zn2+
-
 Bacillus subtilis
Zn2+
-
 Phormidium lapideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADH
-
 Phormidium lapideum
0.04
-
 NAD+
-
 Phormidium lapideum
0.33
-
 pyruvate
-
 Phormidium lapideum
5
-
 L-Ala
-
 Phormidium lapideum
111
-
 NH4+
-
 Phormidium lapideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 6 * 41000, SDS-PAGE Phormidium lapideum
240000
-
 gel filtration Phormidium lapideum

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Phormidium lapideum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phormidium lapideum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
183
-
-
 Phormidium lapideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH 4.1% of the activity with pyruvate Phormidium lapideum 2-aminobutanoate + H2O + NAD+
-
 ?
3-hydroxypyruvate + NH3 + NADH 15.4% of the activity with pyruvate Phormidium lapideum Ser + H2O + NAD+
-
 ?
glyoxylate + NH3 + NADH 1.2% of the activity with pyruvate Phormidium lapideum aminoacetate + NAD+ + H2O
-
 ?
L-Ala + H2O + NAD+
-
 Bacillus subtilis pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Phormidium lapideum pyruvate + NH3 + NADH
-
 r

Subunits

Subunits Comment Organism
hexamer
-
 Bacillus subtilis
hexamer 6 * 41000, SDS-PAGE Phormidium lapideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70 reductive amination Phormidium lapideum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min, pH 6.0-9.0, stable Phormidium lapideum
60
-
 10 min, in presence of 0.3 mM NADH, 30% loss of activity Phormidium lapideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 reductive amination Phormidium lapideum
9.2
-
 oxidative deamination Phormidium lapideum

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.2 pH 7.2: about 40% of maximal activity, pH 9.2: 40% of maximal activity, reductive amination of pyruvate Phormidium lapideum
7.8 10 pH 7.8: about 50% of maximal activity, pH 10.0: about 65% of maximal activity, oxidative deamination of L-Ala Phormidium lapideum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 50°C, 5 min, stable Phormidium lapideum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NAD+
-
 Phormidium lapideum
NADH
-
 Bacillus subtilis
NADH
-
 Phormidium lapideum