Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anoxybacillus flavithermus | - |
- |
- |
[Bacillus] caldolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
[Bacillus] caldolyticus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Anoxybacillus flavithermus | pyruvate + NH3 + NADH | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
enzyme prepared from cells grown at 34°C | Anoxybacillus flavithermus |
60 | - |
enzyme prepared from cells grown at 43°C | Anoxybacillus flavithermus |
62 | - |
enzyme prepared from Bacillus flavothermus cells grown at 52°C or at 70°C | Anoxybacillus flavithermus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
73 | 75 | not active above, temperature maximum depends on growth temperature of cells | Anoxybacillus flavithermus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
stablilized by substrates. Enzyme undergoes a transition from heat-labile to thermostable within the growth temperature range between 44°C and 51°C | Anoxybacillus flavithermus |
70 | - |
5 min, stable in presence of substrates, 20% loss of activity in absence of substrates | Anoxybacillus flavithermus |
70 | - |
5 min, 13% loss of activity in absence of substrates, 2% loss of activity in presence of substrates | [Bacillus] caldolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Bacillus] caldolyticus | |
NAD+ | - |
Anoxybacillus flavithermus | |
NADH | - |
[Bacillus] caldolyticus | |
NADH | - |
Anoxybacillus flavithermus |