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Literature summary for 1.4.1.1 extracted from

  • Lauwers, A.M.; Heinen, W.
    Thermal properties of enzymes from Bacillus flavothermus, grown between 34 and 70¦C (1983), Antonie van Leeuwenhoek, 49, 191-201.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Anoxybacillus flavithermus
-
-
-
[Bacillus] caldolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
[Bacillus] caldolyticus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Anoxybacillus flavithermus pyruvate + NH3 + NADH
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
enzyme prepared from cells grown at 34°C Anoxybacillus flavithermus
60
-
enzyme prepared from cells grown at 43°C Anoxybacillus flavithermus
62
-
enzyme prepared from Bacillus flavothermus cells grown at 52°C or at 70°C Anoxybacillus flavithermus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
73 75 not active above, temperature maximum depends on growth temperature of cells Anoxybacillus flavithermus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
stablilized by substrates. Enzyme undergoes a transition from heat-labile to thermostable within the growth temperature range between 44°C and 51°C Anoxybacillus flavithermus
70
-
5 min, stable in presence of substrates, 20% loss of activity in absence of substrates Anoxybacillus flavithermus
70
-
5 min, 13% loss of activity in absence of substrates, 2% loss of activity in presence of substrates [Bacillus] caldolyticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
[Bacillus] caldolyticus
NAD+
-
Anoxybacillus flavithermus
NADH
-
[Bacillus] caldolyticus
NADH
-
Anoxybacillus flavithermus