Literature summary for 1.4.1.1 extracted from

Ohshima, T.; Wandrey, C.; Sugiura, M.; Soda, K.
Screening of thermostable leucine and alanine dehydrogenases in thermophilic Bacillus strains (1985), Biotechnol. Lett., 7, 871-876.

No PubMed abstract available

Search for more literature for 1.4.1.1

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	screening of thermostable alanine dehydrogenase in thermophilic Bacillus strains	-
Geobacillus stearothermophilus	-	-	-
Lysinibacillus sphaericus	-	-	-
Weizmannia coagulans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala + H2O + NAD+	-	Geobacillus stearothermophilus	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Lysinibacillus sphaericus	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Weizmannia coagulans	pyruvate + NH3 + NADH	-	r
L-Ala + H2O + NAD+	-	Bacillus sp. (in: Bacteria)	pyruvate + NH3 + NADH	-	r

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	screening of thermostable alanine dehydrogenase in thermophilic Bacillus strains	Bacillus sp. (in: Bacteria)
75	-	5 min, stable up to	Geobacillus stearothermophilus
75	-	5 min, stable up to	Lysinibacillus sphaericus
75	-	5 min, stable up to	Weizmannia coagulans
75	-	5 min, stable up to	Bacillus sp. (in: Bacteria)
83	-	5 min, 50% loss of activity	Geobacillus stearothermophilus
83	-	5 min, 50% loss of activity	Lysinibacillus sphaericus
83	-	5 min, 50% loss of activity	Weizmannia coagulans
83	-	5 min, 50% loss of activity	Bacillus sp. (in: Bacteria)

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Geobacillus stearothermophilus
NAD+	-	Lysinibacillus sphaericus
NAD+	-	Weizmannia coagulans
NAD+	-	Bacillus sp. (in: Bacteria)
NADH	-	Geobacillus stearothermophilus
NADH	-	Lysinibacillus sphaericus
NADH	-	Weizmannia coagulans
NADH	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)