Literature summary for 1.4.1.1 extracted from

Vancurova, I.; Vancura, A.; Volc, J.; Neuzil, J.; Flieger, M.; Basarova, G.; Behal, V.
Purification and partial characterization of alanine dehydrogenase from Streptomyces aureofaciens (1988), Arch. Microbiol., 150, 438-440.

No PubMed abstract available

Search for more literature for 1.4.1.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.029	-	NADH	-	Kitasatospora aureofaciens
0.11	-	NAD+	-	Kitasatospora aureofaciens
0.56	-	pyruvate	-	Kitasatospora aureofaciens
5	-	L-Ala	-	Kitasatospora aureofaciens
6.67	-	NH4+	-	Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	8 * 48000, SDS-PAGE	Kitasatospora aureofaciens
395000	-	gel filtration	Kitasatospora aureofaciens

Organism

Organism	UniProt	Comment	Textmining
Kitasatospora aureofaciens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala + H2O + NAD+	-	Kitasatospora aureofaciens	pyruvate + NH3 + NADH	-	r

Subunits

Subunits	Comment	Organism
octamer	8 * 48000, SDS-PAGE	Kitasatospora aureofaciens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Kitasatospora aureofaciens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	reductive amination	Kitasatospora aureofaciens
10	-	oxidative deamination	Kitasatospora aureofaciens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Kitasatospora aureofaciens
NADH	-	Kitasatospora aureofaciens
NADP+	15% of the activity with NAD+	Kitasatospora aureofaciens

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Release 2023.1 (January 2023)