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Literature summary for 1.4.1.1 extracted from
- Continous production of 3-fluoro-L-alanine dehydrogenase (1989), Biotechnol. Bioeng., 34, 394-397.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | continuous production of 3-fluoro-L-alanine with alanine dehydrogenase | Lysinibacillus sphaericus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADH | - |
Lysinibacillus sphaericus |  |
| 0.093 | - |
NADH | - |
Lysinibacillus sphaericus | |
| 1.61 | - |
3-Fluoropyruvate | - |
Lysinibacillus sphaericus | |
| 28.2 | - |
NH3 | - |
Lysinibacillus sphaericus | |
| 1060 | - |
NH3 | - |
Lysinibacillus sphaericus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-fluoropyruvate + NH3 + NADH | - |
Lysinibacillus sphaericus | 3-fluoro-L-alanine + H2O + NAD+ | - |
? | |
| L-Ala + H2O + NAD+ | - |
Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
reaction with 3-fluoropyruvate, NADH and NH4+ | Lysinibacillus sphaericus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Lysinibacillus sphaericus | |
| NADH | - |
Lysinibacillus sphaericus | |
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