Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene insertion mutant is not able to grow on citronellol, geraniol, citronellate, geranylate as sole carbon source | Pseudomonas aeruginosa |
additional information | gene insertion mutant mutants grow slightly more slowly on citronellol and citronellate but show normal growth on geraniol and geranylate | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
citronellyl-CoA | pH 7.0, 30°C | Pseudomonas aeruginosa | |
0.018 | - |
citronellyl-CoA | pH 7.0, 30°C | Pseudomonas aeruginosa | |
0.13 | - |
octanoyl-CoA | pH 7.0, 30°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, SDS-PAGE, x * 42000, calculated | Pseudomonas aeruginosa |
41000 | - |
x * 41000, SDS-PAGE, x * 42700, calculated | Pseudomonas aeruginosa |
42000 | - |
x * 41000, SDS-PAGE, x * 42000, calculated | Pseudomonas aeruginosa |
42700 | - |
x * 41000, SDS-PAGE, x * 42700, calculated | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HZV8 | gene atud; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells | - |
Pseudomonas aeruginosa | Q9I3H8 | gene PA1535, second citronellyl-CoA dehydrogenase, less specific; specific expression in cells growing on acyclic terpenes, absent in isovalerate- or succinate-grown cells | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citronellyl-CoA + 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | no substrates: 5-methylhex-4-enoyl-CoA or isovaleryl-CoA | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no substrates: octanoyl-CoA, 5-methylhex-4-enoyl-CoA or isovaleryl-CoA | Pseudomonas aeruginosa | ? | - |
? | |
octanoyl-CoA + 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | cis-geranyl-CoA + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE, x * 42000, calculated | Pseudomonas aeruginosa |
? | x * 41000, SDS-PAGE, x * 42700, calculated | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | purified and concentrated protein is yellow, and spectral analysis confirms the presence of FAD as cofactor | Pseudomonas aeruginosa |