Cloned (Comment) | Organism |
---|---|
gene KSTD2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally or C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium neoaurum |
General Stability | Organism |
---|---|
both the activity and stability of the enzyme are sensitive to various metal ions | Mycolicibacterium neoaurum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | almost complete inhibition at 1 mM | Mycolicibacterium neoaurum | |
Cu2+ | almost complete inhibition at 1 mM | Mycolicibacterium neoaurum | |
Fe3+ | 40.6% inhibition at 1 mM | Mycolicibacterium neoaurum | |
Mn2+ | almost complete inhibition at 1 mM | Mycolicibacterium neoaurum | |
additional information | not affected by Na+, K+, and EDTA at 1 mM | Mycolicibacterium neoaurum | |
Ni2+ | 59.1% inhibition at 1 mM | Mycolicibacterium neoaurum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Mycolicibacterium neoaurum | |
0.0158 | - |
17alpha,21-dihydroxyprogesterone 21-acetate | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.0211 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.0305 | - |
16,17alpha-epoxyprogesterone | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.0687 | - |
canrenone | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.1434 | - |
4-androstene-3,17-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.1733 | - |
androsta-4,9(11)-diene-3,17-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.1919 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.8327 | - |
9alpha-hydroxy-4-androstene-3,17-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
0.9158 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | slightly activating at 1 mM | Mycolicibacterium neoaurum | |
additional information | both the activity and stability of the enzyme are sensitive to various metal ions | Mycolicibacterium neoaurum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + acceptor | Mycolicibacterium neoaurum | - |
1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | Mycolicibacterium neoaurum DSM 1381 | - |
1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum DSM 1381 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium neoaurum | A0A2P1IUZ5 | - |
- |
Mycolicibacterium neoaurum DSM 1381 | A0A2P1IUZ5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally or C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Mycolicibacterium neoaurum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1586 | - |
purified enzyme, pH 8.0, 40°C, substrate 4-androstene-3,17-dione, conversion rate is 99% | Mycolicibacterium neoaurum |
1719.21 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 9alpha-hydroxy-4-androstene-3,17-dione | Mycolicibacterium neoaurum |
2560.15 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate canrenone | Mycolicibacterium neoaurum |
2623.11 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | Mycolicibacterium neoaurum |
5975.86 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate androsta-4,9(11)-diene-3,17-dione | Mycolicibacterium neoaurum |
6299.29 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | Mycolicibacterium neoaurum |
7800.55 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 17alpha,21-dihydroxyprogesterone 21-acetate | Mycolicibacterium neoaurum |
8882.95 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 4-androstene-3,17-dione | Mycolicibacterium neoaurum |
9163.63 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 17alpha-hydroxypregn-4-ene-3,20-dione | Mycolicibacterium neoaurum |
12781.5 | - |
pH 8.0, 40°C, purified recombinant enzyme, substrate 6,17alpha-epoxyprogesterone | Mycolicibacterium neoaurum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor | hydrocortisone | Mycolicibacterium neoaurum | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor | hydrocortisone | Mycolicibacterium neoaurum DSM 1381 | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone acetate | Mycolicibacterium neoaurum | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone acetate | Mycolicibacterium neoaurum DSM 1381 | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
16,17alpha-epoxyprogesterone + acceptor | - |
Mycolicibacterium neoaurum | ? + reduced acceptor | - |
? | |
17alpha,21-dihydroxyprogesterone 21-acetate + acceptor | RSA | Mycolicibacterium neoaurum | ? + reduced acceptor | - |
? | |
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor | - |
Mycolicibacterium neoaurum | 1,4-pregnene-17alpha-ol-3,20-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium neoaurum | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium neoaurum DSM 1381 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium neoaurum | ? + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum DSM 1381 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
androsta-4,9(11)-diene-3,17-dione + acceptor | NSC 44826 | Mycolicibacterium neoaurum | ? + reduced acceptor | - |
? | |
canrenone + acceptor | - |
Mycolicibacterium neoaurum | ? + reduced acceptor | - |
? | |
additional information | enzyme KstD2 displays a high activity on a range of substrates, including 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-OH-P), androsta-4,9(11)-diene-3,17-dione (NSC 44826), and 4-androstene-3,17-dione (AD), substrate activity and kinetics, overview | Mycolicibacterium neoaurum | ? | - |
- |
|
additional information | enzyme KstD2 displays a high activity on a range of substrates, including 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-OH-P), androsta-4,9(11)-diene-3,17-dione (NSC 44826), and 4-androstene-3,17-dione (AD), substrate activity and kinetics, overview | Mycolicibacterium neoaurum DSM 1381 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid-DELTA1-dehydrogenase | - |
Mycolicibacterium neoaurum |
KSTD2 | - |
Mycolicibacterium neoaurum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Mycolicibacterium neoaurum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | over 50% of maximal activity within this range | Mycolicibacterium neoaurum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant enzyme, completely stable at | Mycolicibacterium neoaurum |
40 | - |
purified recombinant enzyme, 80% activity remaining | Mycolicibacterium neoaurum |
45 | - |
purified recombinant enzyme, 20% activity remaining | Mycolicibacterium neoaurum |
50 | - |
purified recombinant enzyme, inactivation | Mycolicibacterium neoaurum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1862.9 | - |
9alpha-hydroxy-4-androstene-3,17-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
2774.12 | - |
canrenone | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
2842.34 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
6475.3 | - |
androsta-4,9(11)-diene-3,17-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
6825.77 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
8452.5 | - |
17alpha,21-dihydroxyprogesterone 21-acetate | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
9625.36 | - |
4-androstene-3,17-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
9929.5 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum | |
13849.8 | - |
16,17alpha-epoxyprogesterone | recombinant enzyme, pH 8.0, 40°C | Mycolicibacterium neoaurum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycolicibacterium neoaurum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | over 50% of maximal activity within this range | Mycolicibacterium neoaurum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
purified recombinant enzyme, 25% activity remaining | Mycolicibacterium neoaurum |
6 | 9 | purified recombinant enzyme, over 80% activity remaining | Mycolicibacterium neoaurum |
10 | - |
purified recombinant enzyme, 30% activity remaining | Mycolicibacterium neoaurum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mycolicibacterium neoaurum |
General Information | Comment | Organism |
---|---|---|
additional information | homology-based structural analysis and structure modeling of KstD2 using the structure of SQ1-KstD1 Rhodococcus erythropolis SQ1 (PDB ID 4C3Y). Enzyme KstD1 and KstD2 belongs to clusters 1 and 2, respectively | Mycolicibacterium neoaurum |
physiological function | 3-ketosteroid-DELTA1-dehydrogenase (KstD) catalyzes DELTA1-dehydrogenation and is involved in the steroid catabolism | Mycolicibacterium neoaurum |