Application | Comment | Organism |
---|---|---|
synthesis | recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization | Rhodococcus erythropolis |
synthesis | recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
gene ksdD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium neoaurum |
gene kstd, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodococcus erythropolis |
gene kstd, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium neoaurum |
gene kstd, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
gene kstd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
gene kstd3, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0123 | - |
cortexolone | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.014 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0178 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0182 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0193 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0202 | - |
progesterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0226 | - |
testosterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0232 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0272 | - |
cortisone | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0369 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0373 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0401 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0463 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0481 | - |
4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0509 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
0.0667 | - |
progesterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0707 | - |
testosterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.075 | - |
pregna-4,9(11),16-triene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0853 | - |
cortisone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0917 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0933 | - |
cortexolone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.0985 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.1003 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.1385 | - |
4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.2102 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.2121 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
0.2462 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 30°C | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3-oxosteroid + acceptor | Rhodococcus erythropolis | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium smegmatis | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Rhodococcus erythropolis WY 1406 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Rhodococcus erythropolis CGMCC 5096 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum 2967 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium neoaurum 2966 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium smegmatis ATCC 700084 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | Mycolicibacterium smegmatis mc2155 | - |
a 3-oxo-delta1-steroid + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium neoaurum | - |
- |
- |
Mycolicibacterium neoaurum | C6KID9 | - |
- |
Mycolicibacterium neoaurum | C8CJG6 | - |
- |
Mycolicibacterium neoaurum 2966 | - |
- |
- |
Mycolicibacterium neoaurum 2966 | C6KID9 | - |
- |
Mycolicibacterium neoaurum 2966 | C8CJG6 | - |
- |
Mycolicibacterium neoaurum 2967 | C6KID9 | - |
- |
Mycolicibacterium smegmatis | A0QW99 | - |
- |
Mycolicibacterium smegmatis | A0R1T2 | - |
- |
Mycolicibacterium smegmatis | A0R1T7 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0QW99 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0R1T2 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0R1T7 | - |
- |
Mycolicibacterium smegmatis mc2155 | A0QW99 | - |
- |
Mycolicibacterium smegmatis mc2155 | A0R1T2 | - |
- |
Mycolicibacterium smegmatis mc2155 | A0R1T7 | - |
- |
Rhodococcus erythropolis | Q9RA02 | - |
- |
Rhodococcus erythropolis CGMCC 5096 | Q9RA02 | - |
- |
Rhodococcus erythropolis WY 1406 | Q9RA02 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodococcus erythropolis |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme, stable in 50 mM Tris-HCl, pH 8.0, for at least 2 weeks | Rhodococcus erythropolis |
4°C, purified recombinant enzyme, stable in 50 mM Tris-HCl, pH 8.0, for at least 2 weeks | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone acetate | Rhodococcus erythropolis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor | hydrocortisone acetate | Mycolicibacterium smegmatis | 1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor | - |
? | |
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
4-androsten-3-one-5-ene-17-carboxylic acid + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
4-androsten-3-one-5-ene-17-carboxylic acid + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis WY 1406 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis CGMCC 5096 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis ATCC 700084 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis mc2155 | 1,4-androstadiene-3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis WY 1406 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Rhodococcus erythropolis CGMCC 5096 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis ATCC 700084 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
9alpha-hydroxy-4-androstene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis mc2155 | 9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Rhodococcus erythropolis | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium smegmatis | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Rhodococcus erythropolis WY 1406 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Rhodococcus erythropolis CGMCC 5096 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum 2967 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium neoaurum 2966 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium smegmatis ATCC 700084 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
a 3-oxosteroid + acceptor | - |
Mycolicibacterium smegmatis mc2155 | a 3-oxo-delta1-steroid + reduced acceptor | - |
? | |
androst-4,9(11)-diene-3,17-dione + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
androst-4,9(11)-diene-3,17-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
cortexolone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cortexolone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cortisone + acceptor | - |
Rhodococcus erythropolis | ? + reduced acceptor | - |
? | |
cortisone + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
additional information | KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization | Rhodococcus erythropolis | ? | - |
- |
|
additional information | the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave | Mycolicibacterium smegmatis | ? | - |
- |
|
additional information | KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization | Rhodococcus erythropolis WY 1406 | ? | - |
- |
|
additional information | KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization | Rhodococcus erythropolis CGMCC 5096 | ? | - |
- |
|
additional information | the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave | Mycolicibacterium smegmatis ATCC 700084 | ? | - |
- |
|
additional information | the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave | Mycolicibacterium smegmatis mc2155 | ? | - |
- |
|
pregna-4,9(11),16-triene-3,20-dione + acceptor | - |
Mycolicibacterium smegmatis | ? + reduced acceptor | - |
? | |
progesterone + acceptor | - |
Rhodococcus erythropolis | pregna-1,4-diene-3,20-dione + reduced acceptor | - |
? | |
progesterone + acceptor | - |
Mycolicibacterium smegmatis | pregna-1,4-diene-3,20-dione + reduced acceptor | - |
? | |
testosterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
testosterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
testosterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis WY 1406 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
testosterone + 2,6-dichlorophenol-indophenol | - |
Rhodococcus erythropolis CGMCC 5096 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
testosterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis ATCC 700084 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? | |
testosterone + 2,6-dichlorophenol-indophenol | - |
Mycolicibacterium smegmatis mc2155 | 17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53800, recombinant enzyme, SDS-PAGE, x * 53073, sequence calculation | Rhodococcus erythropolis |
? | x * 55200, recombinant enzyme, SDS-PAGE, x * 54806, sequence calculation | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid-DELTA1-dehydrogenase | - |
Rhodococcus erythropolis |
3-ketosteroid-DELTA1-dehydrogenase | - |
Mycolicibacterium smegmatis |
KsdD | - |
Mycolicibacterium neoaurum |
KstD | - |
Rhodococcus erythropolis |
KstD | - |
Mycolicibacterium smegmatis |
MnKstD1 | - |
Mycolicibacterium neoaurum |
MnKstD2 | - |
Mycolicibacterium neoaurum |
MsKstD1 | - |
Mycolicibacterium smegmatis |
MsKstD2 | - |
Mycolicibacterium smegmatis |
MsKstD3 | - |
Mycolicibacterium smegmatis |
MsKstD4 | - |
Mycolicibacterium neoaurum |
ReKstD1 | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
recombinant enzyme | Mycolicibacterium smegmatis |
30 | - |
assay at | Mycolicibacterium neoaurum |
30 | - |
assay at | Mycolicibacterium smegmatis |
30 | - |
recombinant enzyme | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.2 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
12.8 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
13.7 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
14.4 | - |
pregna-4,9(11),16-triene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
20.3 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 30°C | Rhodococcus erythropolis | |
20.6 | - |
cortexolone | pH 8.0, 30°C | Rhodococcus erythropolis | |
21.4 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
21.4 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
22.3 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
24.6 | - |
cortisone | pH 8.0, 30°C | Rhodococcus erythropolis | |
25.4 | - |
progesterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
25.8 | - |
testosterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
26.9 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 30°C | Rhodococcus erythropolis | |
40.8 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
47.5 | - |
4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
50.2 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
51.5 | - |
4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
52.2 | - |
cortisone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
58.7 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
60.3 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
63.5 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
77.2 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
77.5 | - |
testosterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
94.1 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
94.2 | - |
cortexolone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
110.4 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
127.8 | - |
progesterone | pH 8.0, 25°C | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Rhodococcus erythropolis |
8 | - |
recombinant enzyme | Mycolicibacterium smegmatis |
8 | - |
assay at | Mycolicibacterium neoaurum |
8 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | - |
Rhodococcus erythropolis | |
2,6-dichlorophenolindophenol | - |
Mycolicibacterium smegmatis | |
phenazine methosulfate | - |
Rhodococcus erythropolis | |
phenazine methosulfate | preferred cofactor | Mycolicibacterium smegmatis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | 12 | cortisone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
48.1 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
165.7 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 30°C | Rhodococcus erythropolis | |
192 | - |
pregna-4,9(11),16-triene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
367.3 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
371.8 | - |
4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
420.4 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
447.7 | - |
4-androsten-3-one-5-ene-17-carboxylic acid | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
644.7 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
663.2 | - |
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
769.7 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
875 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate | pH 8.0, 30°C | Rhodococcus erythropolis | |
904.4 | - |
cortisone | pH 8.0, 30°C | Rhodococcus erythropolis | |
987.5 | - |
4-androstene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
1009.6 | - |
cortexolone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
1084.2 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
1096.2 | - |
testosterone | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
1141.6 | - |
testosterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
1175.8 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
1203.9 | - |
17alpha-hydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
1257.4 | - |
progesterone | pH 8.0, 30°C | Rhodococcus erythropolis | |
1503.7 | - |
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
1511.2 | - |
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone | pH 8.0, 30°C | Rhodococcus erythropolis | |
1590.8 | - |
9alpha-hydroxy-4-androstene-3,17-dione | pH 8.0, 25°C | Mycolicibacterium smegmatis | |
1592.9 | - |
androst-4,9(11)-diene-3,17-dione | pH 8.0, 30°C | Rhodococcus erythropolis | |
1674.8 | - |
cortexolone | pH 8.0, 30°C | Rhodococcus erythropolis | |
1916 | - |
progesterone | pH 8.0, 25°C | Mycolicibacterium smegmatis |