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Literature summary for 1.3.99.4 extracted from

  • Brzezinska, M.; Szulc, I.; Brzostek, A.; Klink, M.; Kielbik, M.; Sulowska, Z.; Pawelczyk, J.; Dziadek, J.
    The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis (2013), BMC Microbiol., 13, 43.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.99.4

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
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 gene kstD
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Mycobacterium tuberculosis H37Rv
-
 gene kstD
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Synonyms

Synonyms Comment Organism
3-ketosteroid 1(2)-dehydrogenase
-
 Mycobacterium tuberculosis
KstD
-
 Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis construction of a mutant Mtb H37Rv strain containing an inactivated kstD gene, DELTAkstD, which encodes 3-ketosteroid 1(2)-dehydrogenase by homologous recombination-based gene-replacement technique. Replication of mutant Mycobacterium tuberculosis is attenuated in resting human macrophages compared to the wild-type or complemented strains. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages. But mutant and wild-type Mycobacterium tuberculosis behave similarly in macrophages activated with IFN-gamma before and during infection. The mutant is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply additional information

General Information

General Information Comment Organism
malfunction an enzyme-deficient mutant strain is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages, phenotpe, overview Mycobacterium tuberculosis