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Literature summary for 1.3.99.31 extracted from
- Expression in Escherichia coli, purification, and reactivation of the recombinant Erwinia uredovora phytoene desaturase (1992), J. Biol. Chem., 267, 19891-19895.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pantoea ananatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pantoea ananatis |  |
| NADP+ | - |
Pantoea ananatis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Pantoea ananatis | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56200 | - |
x * 56200, SDS-PAGE | Pantoea ananatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 15-cis-phytoene + 4 acceptor | Pantoea ananatis | overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed | all-trans-lycopene + 4 reduced acceptor | - |
? | |
| all-trans-neurosporene + acceptor | Pantoea ananatis | - |
all-trans-lycopene + reduced acceptor | - |
? | |
| all-trans-phytofluene + 3 acceptor | Pantoea ananatis | - |
all-trans-lycopene + 3 reduced acceptor | - |
? | |
| all-trans-zeta-carotene + 2 acceptor | Pantoea ananatis | - |
all-trans-lycopene + 2 reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pantoea ananatis | P21685 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pantoea ananatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 15-cis-phytoene + 4 acceptor | overall reaction. all-trans-Lycopene is the principal desaturase product constituting 60% of the total reaction products. The desaturase intermediates all-trans-phytofluene and all-trans-zeta-carotene are also detected and constitute 27 and 12% of the total desaturase products formed | Pantoea ananatis | all-trans-lycopene + 4 reduced acceptor | - |
? | |
| all-trans-neurosporene + acceptor | - |
Pantoea ananatis | all-trans-lycopene + reduced acceptor | - |
? | |
| all-trans-phytofluene + 3 acceptor | - |
Pantoea ananatis | all-trans-lycopene + 3 reduced acceptor | - |
? | |
| all-trans-zeta-carotene + 2 acceptor | - |
Pantoea ananatis | all-trans-lycopene + 2 reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56200, SDS-PAGE | Pantoea ananatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CrtI | - |
Pantoea ananatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pantoea ananatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | involved in desaturation. FAD is a dissociable moiety and not covalently bound to the enzyme | Pantoea ananatis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in carotenoid biosynthesis | Pantoea ananatis |
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