Protein Variants | Comment | Organism |
---|---|---|
F166I | mutation changes the product of phytoene desaturation from neurosporene to lycopene | Cereibacter sphaeroides |
H12Q | mutation has little effect on the product formation | Cereibacter sphaeroides |
M402T | mutation has a negative effect on percent lycopene production | Cereibacter sphaeroides |
additional information | directed evolution is used to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene. Two generations of random mutagenesis are performed, from which three positive mutants are isolated and sequenced. Site-directed mutagenesis is used to determine the effect of each amino acid change | Cereibacter sphaeroides |
V68D | mutation changes the product of phytoene desaturation from neurosporene to lycopene | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 3 acceptor | Cereibacter sphaeroides | the enzyme is involved in carotenoid biosynthesis | all-trans-neurosporene + 3 reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 3 acceptor | the enzyme is involved in carotenoid biosynthesis | Cereibacter sphaeroides | all-trans-neurosporene + 3 reduced acceptor | - |
? | |
phytoene + acceptor | - |
Cereibacter sphaeroides | neurosporene + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CrtI | - |
Cereibacter sphaeroides |
General Information | Comment | Organism |
---|---|---|
physiological function | involved in carotenoid biosynthesis | Cereibacter sphaeroides |