Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0134 | - |
1-hydroxyneurosporene | pH 8.0, 30°C | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1'-hydroxy-gamma-carotene + acceptor | - |
Cereibacter sphaeroides | 1'-hydroxytorulene + reduced acceptor | - |
? | |
1-hydroxylycopene + acceptor | - |
Cereibacter sphaeroides | 1-hydroxy-3,4-didehydrolycopene + reduced acceptor | - |
? | |
1-hydroxyneurosporene + acceptor | when O2 is depleted less than 10% of the specific activity is retained. It is rather unlikely that molecular oxygen is the genuine cofactor for hydroxyneurosporene desaturation reactions in Rhodobacter cells when they reach their maximum carotenoid content under anaerobic conditions. Consequently, another, as-yet-unknown electron acceptor may exist in Rhodobacter species that replaces molecular oxygen during anaerobic growth | Cereibacter sphaeroides | demethylspheroidene + reduced acceptor | - |
? | |
additional information | 1'-hydroxy-3,4-didehydrolycopene and 1,1'-dihydroxylycopene are poor substrates. 1-Methoxyneurosporene is not converted by purified hydroxyneurosporene desaturase | Cereibacter sphaeroides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NAD+, NADP+, and flavin adenine dinucleotide have no influence on enzymatic activity. When O2 is depleted less than 10% of the specific activity is retained. It is rather unlikely that molecular oxygen is the genuine cofactor for hydroxyneurosporene desaturation reactions in Rhodobacter cells when they reach their maximum carotenoid content under anaerobic conditions. Consequently, another, as-yet-unknown electron acceptor may exist in Rhodobacter species that replaces molecular oxygen during anaerobic growth | Cereibacter sphaeroides |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the carotenoid biosynthetic pathway of Rhodobacter species | Cereibacter sphaeroides |