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Literature summary for 1.3.99.27 extracted from

  • Steiger, S.; Astier, C.; Sandmann, G.
    Substrate specificity of the expressed carotenoid 3,4-desaturase from Rubrivivax gelatinosus reveals the detailed reaction sequence to spheroidene and spirilloxanthin (2000), Biochem. J., 349, 635-640.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rubrivivax gelatinosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
1,1'-dihydroxyneurosporene pH 7.8, 28°C Rubrivivax gelatinosus
0.025
-
1-hydroxyneurosporene pH 7.8, 28°C Rubrivivax gelatinosus
0.04
-
1-hydroxylycopene pH 7.8, 28°C Rubrivivax gelatinosus

Organism

Organism UniProt Comment Textmining
Rubrivivax gelatinosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1'-hydroxy-gamma-carotene + acceptor 15% conversion Rubrivivax gelatinosus 1'-hydroxytorulene + reduced acceptor
-
?
1,1'-dihydroxy-3,4-didehydrolycopene + acceptor 38% conversion Rubrivivax gelatinosus 1,1'-dihydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
?
1,1'-dihydroxyneurosporene + acceptor 39% conversion Rubrivivax gelatinosus 1'-hydroxy-1-demethylspheroidene + reduced acceptor
-
?
1-hydroxy-3',4'-didehydrolycopene + acceptor 28% conversion Rubrivivax gelatinosus 1-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
?
1-hydroxy-zeta-carotene + acceptor 15% conversion Rubrivivax gelatinosus 1-hydroxy-3,4-didehydro-zeta-carotene + reduced acceptor
-
?
1-hydroxylycopene + acceptor 33% conversion. The 3,4-desaturation reaction can only occur with mono-1-hydroxy carotenoids at a Psi-end group or with 1,1'-dihydroxy derivatives carrying a 3'4'-double bond. In addition, 1-hydroxy-zeta-carotene could also be converted by the desaturase. Enzyme kinetic studies show a substrate preference of 1-hydroxyneurosporene over 1-hydroxyycopene Rubrivivax gelatinosus 1-hydroxy-3,4-didehydrolycopene + reduced acceptor
-
?
1-hydroxyneurosporene + acceptor 47% conversion. The 3,4-desaturation reaction can only occur with mono-1-hydroxy carotenoids at a Psi-end group or with 1,1'-dihydroxy derivatives carrying a 3'4'-double bond. In addition, 1-hydroxy-zeta-carotene could also be converted by the desaturase. Enzyme kinetic studies show a substrate preference of 1-hydroxyneurosporene over 1-hydroxylycopene Rubrivivax gelatinosus 1-demethylspheroidene + reduced acceptor
-
?
1-methoxy-1'-hydroxy-3,4-didehydrolycopene + acceptor 37% conversion Rubrivivax gelatinosus 1-methoxy-1'-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
?
additional information no activity with 1,1'-dihydroxylycopene, 1-methoxyneurosporene, 1'-hydroxydemethylspheroidene, 1,1'-dihydroxy-zeta-carotene, demethylspheroidene and 1'-hydroxyspheroidene Rubrivivax gelatinosus ?
-
?

Synonyms

Synonyms Comment Organism
CrtD
-
Rubrivivax gelatinosus
hydroxyneurosporene desaturase
-
Rubrivivax gelatinosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Rubrivivax gelatinosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Rubrivivax gelatinosus

General Information

General Information Comment Organism
malfunction the deletion mutant shows a different carotenoid pattern than the wild-type enzyme Rubrivivax gelatinosus
physiological function the enzyme is involved in biosynthesis of spirilloxanthin and spheroidene Rubrivivax gelatinosus