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Literature summary for 1.3.99.23 extracted from

  • Chen, Y.; Reese, D.H.
    The retinol signaling pathway in mouse pluripotent P19 cells (2011), J. Cell. Biochem., 112, 2865-2872.
    View publication on PubMed

Application

Application Comment Organism
analysis the mouse pluripotent P-19 cell metabolizes retinol to atRA and thus can be used in a cell-based screen for disruptors of the pathway. The disruption of the pathway is easily detected and quantitated, the P-19 cell provides an in vitro model system for identifying and exploring the mechanism of action of chemicals that interfere with the critical cellular pathway Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
quantitative real-time PCR expression analysis Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-13,14-dihydroretinol + acceptor Mus musculus
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all-trans-retinol + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
P-19 cell pluripotent embryonal carcinoma cells with high enzyme mRNA level, quantitative real-time PCR expression analysis Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-13,14-dihydroretinol + acceptor
-
Mus musculus all-trans-retinol + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
13,14-dihydroretinol saturase
-
Mus musculus
all-trans-13,14-dihydroretinol saturase
-
Mus musculus
RetSat
-
Mus musculus

Expression

Organism Comment Expression
Mus musculus effect of retinol on gene expressions in P-19 cells, overview additional information

General Information

General Information Comment Organism
metabolism retinol signaling plays an important role in the establishment and maintenance of cellular phenotype in embryonic and adult vertebrate tissues. all-trans-Retinoic acid functions as the activating ligand for a family of ligand-activated transcription factors, the retinoic acid receptors, which form heterodimers with the retinoid X receptors to regulate gene transcription. Through its activation of the receptors, all-trans-retinoic acid regulates the expression of over 500 protein-coding genes Mus musculus
additional information the mouse pluripotent P-19 cell metabolizes retinol to all-trans-retinoic acid, analysis of expression of enzymes in the cell involved in the pathway, overview Mus musculus