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Literature summary for 1.3.8.8 extracted from

  • Hall, C.L.; Heijkenskjöld, L.; Bartfai, T.; Ernster, L.; Kamin, H.
    Acyl coenzyme A dehydrogenases and electron-transferring flavoprotein from beef hart mitochondria (1976), Arch. Biochem. Biophys., 177, 402-414.
    View publication on PubMed
Search for more literature for 1.3.8.8

Inhibitors

Inhibitors Comment Organism Structure
palmitoyl-CoA inhibition by micelle formation at concentrations up to 0.004 mM, reversed by fatty acid free bovine serum albumin Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 palmitoyl-CoA
-
 Bos taurus
0.001
-
 palmitoyl-CoA in the presence of fatty acid free bovine serum albumin Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
40-75% ammonium sulfate precipitation, G-200 electrophoresis Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.076
-
 purified enzyme, butyryl-CoA Bos taurus
0.19
-
 purified enzyme, palmitoyl-CoA, 0.12 nmol/ml ETF Bos taurus
0.259
-
 purified enzyme, palmitoyl-CoA, 0.24 nmol/ml ETF Bos taurus
0.272
-
 purified enzyme, octanoyl-CoA Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyryl-CoA + electron-transfer flavoprotein
-
 Bos taurus 2-butenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
octanoyl-CoA + electron-transfer flavoprotein
-
 Bos taurus 2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
palmitoyl-CoA + electron-transfer flavoprotein
-
 Bos taurus 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 38000-45200, SDS-PAGE Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.67
-
 palmitoyl-CoA saturating ETF Bos taurus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Bos taurus