Literature summary for 1.3.8.7 extracted from

Nandy, A.; Kieweg, V.; Kraeutle, F.G.; Vock, P.; Kuechler, B.; Bross, P.; Kim, J.J.P.; Rasched, I.; Ghisla, S.
Medium-long-chain chimeric human acyl-CoA dehydrogenase: medium-chain enzyme with the active center base arrangement of long-chain acyl-CoA dehydrogenase (1996), Biochemistry, 35, 12402-12411.

Search for more literature for 1.3.8.7

Protein Variants

Protein Variants	Comment	Organism
T255E	same activity with octanoyl-CoA as wild-type, activity/chain length profile is, however, narrower	Homo sapiens
T255E/E376G	chimeric medium-long chain acyl-CoA dehydrogenase, has 20% of the activity of medium-chain acyl-CoA dehydrogenase and 25% that of long-chain acyl-CoA dehydrogenase activity with its best substrates octanoyl-CoA and dodecanoyl-CoA respectively, activity maximum is shifted to C12 and C14-CoA, enzyme shows an enhanced rate of reoxidation with oxygen	Homo sapiens
T255E/E376T	shifts the chain length activity profile to higher values, drastic reduction of maximal velocity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.001	-	octanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.0013	-	hexadecanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.0014	-	hexanoyl-CoA	T255E mutant enzyme	Homo sapiens
0.0016	-	hexadecanoyl-CoA	-	Homo sapiens
0.0023	-	tetradecanoyl-CoA	-	Homo sapiens
0.0025	-	decanoyl-CoA	-	Homo sapiens
0.0025	-	dodecanoyl-CoA	-	Homo sapiens
0.0034	-	octanoyl-CoA	-	Homo sapiens
0.0042	-	decanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.0055	-	tetradecanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.0066	-	dodecanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.008	-	octanoyl-CoA	T255E mutant enzyme	Homo sapiens
0.01	-	decanoyl-CoA	T255E mutant enzyme	Homo sapiens
0.015	-	hexanoyl-CoA	-	Homo sapiens
0.05	-	decanoyl-CoA	T255E/E376T double mutant enzyme	Homo sapiens
0.05	-	dodecanoyl-CoA	T255E/E376T double mutant enzyme	Homo sapiens
0.07	-	butanoyl-CoA	T255E mutant enzyme	Homo sapiens
0.175	-	butanoyl-CoA	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type, T255E, T255E/E367G and T255E/E367T mutant enzymes	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
a medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein	the catalytic base responsible for the alpha-proton abstraction is E376 in medium-chain acyl-CoA dehydrogenase, while that in long-chain acyl-CoA dehydrogenase is E255	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butanoyl-CoA + acceptor	-	Homo sapiens	crotonyl-CoA + reduced acceptor	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.167	-	octanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.233	-	decanoyl-CoA	T255E/E376T double mutant enzyme	Homo sapiens
0.333	-	decanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
0.617	-	hexadecanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
2.33	-	hexadecanoyl-CoA	-	Homo sapiens
2.67	-	tetradecanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
2.83	-	hexanoyl-CoA	T255E mutant enzyme	Homo sapiens
3.33	-	dodecanoyl-CoA	T255E/E376G double mutant enzyme	Homo sapiens
4.67	-	tetradecanoyl-CoA	-	Homo sapiens
8.33	-	butanoyl-CoA	wild-type and T255E mutant enzyme	Homo sapiens
9.17	-	dodecanoyl-CoA	-	Homo sapiens
9.67	-	decanoyl-CoA	-	Homo sapiens
11.3	-	decanoyl-CoA	T255E mutant enzyme	Homo sapiens
13.2	-	octanoyl-CoA	T255E mutant enzyme	Homo sapiens
16.3	-	octanoyl-CoA	-	Homo sapiens
16.8	-	hexanoyl-CoA	-	Homo sapiens

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Release 2023.1 (January 2023)