Crystallization (Comment) | Organism |
---|---|
purified GDH, hanging drop vapor diffusion method, 10 mg/ml protein in 10 mM MES, pH 6.0, 0.5 M KCl, 10% w/v glycerol, 1 mM DTT, 1 mM FAD, and glutaryl-CoA, are mixed with an equal volume of reservoir solution containing 15% v/v MPD, 0.1 M imidazole, pH 6.5, 0.2 M KCl, and 2% PEG 3350, at 4°C, 2 days, X-ray diffraction structure determination and analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + FAD | Homo sapiens | - |
crotonoyl-CoA + CO2 + FADH2 | - |
? | |
additional information | Homo sapiens | the decarboxylating and nondecarboxylating, EC 1.3.99.X from Desulfococcus multivorans, capabilities are provided by complex structural changes around the glutaconyl carboxylate group, the key factor being a Tyr to Val exchange strictly conserved between the two GDH types | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q92947 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + FAD | - |
Homo sapiens | crotonoyl-CoA + CO2 + FADH2 | - |
? | |
glutaryl-CoA + FAD | detection using ferrocenium hexafluorophosphate | Homo sapiens | crotonoyl-CoA + CO2 + FADH2 | - |
? | |
additional information | the decarboxylating and nondecarboxylating, EC 1.3.99.X from Desulfococcus multivorans, capabilities are provided by complex structural changes around the glutaconyl carboxylate group, the key factor being a Tyr to Val exchange strictly conserved between the two GDH types | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure determination and comparison of carboxylating GDH to the Desulfococcus multivorans nondecarboxylating GDH, EC 1.3.99.X, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Homo sapiens |
glutaryl-coenzyme A dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | glutaryl-coenzyme A dehydrogenases involved in amino acid degradation catalyze both the dehydrogenation and decarboxylation of glutaryl-CoA to crotonoyl-CoA and CO2 | Homo sapiens |