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Literature summary for 1.3.8.6 extracted from

  • Wischgoll, S.; Demmer, U.; Warkentin, E.; Guenther, R.; Boll, M.; Ermler, U.
    Structural basis for promoting and preventing decarboxylation in glutaryl-coenzyme A dehydrogenases (2010), Biochemistry, 49, 5350-5357.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified GDH, hanging drop vapor diffusion method, 10 mg/ml protein in 10 mM MES, pH 6.0, 0.5 M KCl, 10% w/v glycerol, 1 mM DTT, 1 mM FAD, and glutaryl-CoA, are mixed with an equal volume of reservoir solution containing 15% v/v MPD, 0.1 M imidazole, pH 6.5, 0.2 M KCl, and 2% PEG 3350, at 4°C, 2 days, X-ray diffraction structure determination and analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutaryl-CoA + FAD Homo sapiens
-
crotonoyl-CoA + CO2 + FADH2
-
?
additional information Homo sapiens the decarboxylating and nondecarboxylating, EC 1.3.99.X from Desulfococcus multivorans, capabilities are provided by complex structural changes around the glutaconyl carboxylate group, the key factor being a Tyr to Val exchange strictly conserved between the two GDH types ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92947
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-CoA + FAD
-
Homo sapiens crotonoyl-CoA + CO2 + FADH2
-
?
glutaryl-CoA + FAD detection using ferrocenium hexafluorophosphate Homo sapiens crotonoyl-CoA + CO2 + FADH2
-
?
additional information the decarboxylating and nondecarboxylating, EC 1.3.99.X from Desulfococcus multivorans, capabilities are provided by complex structural changes around the glutaconyl carboxylate group, the key factor being a Tyr to Val exchange strictly conserved between the two GDH types Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More structure determination and comparison of carboxylating GDH to the Desulfococcus multivorans nondecarboxylating GDH, EC 1.3.99.X, overview Homo sapiens

Synonyms

Synonyms Comment Organism
GDH
-
Homo sapiens
glutaryl-coenzyme A dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens

General Information

General Information Comment Organism
metabolism glutaryl-coenzyme A dehydrogenases involved in amino acid degradation catalyze both the dehydrogenation and decarboxylation of glutaryl-CoA to crotonoyl-CoA and CO2 Homo sapiens