Cloned (Comment) | Organism |
---|---|
expression of liuA in Escherichia coli strain Rosetta 2 (DE3), subcloning in Escherichia coli strain JM109 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of insertion mutants, which show completely impaired growth on all acyclic terpenes tested, i.e.citronellol, geraniol, sodium salts of citronellate and geranylate, and on leucine and isovalerate, phenotype, overview | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
isovaleryl-CoA | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation | Pseudomonas aeruginosa |
44000 | - |
4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation | Pseudomonas aeruginosa |
163000 | - |
recombinant enzyme, gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + 2,6-dichlorophenolindophenol | Pseudomonas aeruginosa | high substrate specificity | ? | - |
? | |
additional information | Pseudomonas aeruginosa | the enzyme is required in the leucine/isovalerate utilization, Liu, pathway for growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate, Liu proteins in metabolism of methyl-branched compounds, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene liuA as part of the liuABCDE gene cluster | - |
Purification (Comment) | Organism |
---|---|
recombinant LiuA from Escherichia coli | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + 2,6-dichlorophenolindophenol | high substrate specificity | Pseudomonas aeruginosa | ? | - |
? | |
isovaleryl-CoA + 2,6-dichlorophenolindophenol | high substrate specificity | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme is required in the leucine/isovalerate utilization, Liu, pathway for growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate, Liu proteins in metabolism of methyl-branched compounds, overview | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no activity of LiuA with isobutyryl-CoA, 3-hydroxybutyryl-CoA, octanoyl-CoA, citronellyl-CoA or 5-methyl-hex-4-enoyl-CoA | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA dehydrogenase | - |
Pseudomonas aeruginosa |
LiuA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | |
FAD | - |
Pseudomonas aeruginosa |