Any feedback?
Literature summary for 1.3.8.4 extracted from
- Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes for a functional catabolic pathway of methyl-branched compounds (2008), FEMS Microbiol. Lett., 286, 78-84.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of liuA in Escherichia coli strain Rosetta 2 (DE3), subcloning in Escherichia coli strain JM109 | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of insertion mutants, which show completely impaired growth on all acyclic terpenes tested, i.e.citronellol, geraniol, sodium salts of citronellate and geranylate, and on leucine and isovalerate, phenotype, overview | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
isovaleryl-CoA | pH 7.5, 30°C, recombinant enzyme | Pseudomonas aeruginosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation | Pseudomonas aeruginosa |
| 44000 | - |
4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation | Pseudomonas aeruginosa |
| 163000 | - |
recombinant enzyme, gel filtration | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isovaleryl-CoA + 2,6-dichlorophenolindophenol | Pseudomonas aeruginosa | high substrate specificity | ? | - |
? | |
| additional information | Pseudomonas aeruginosa | the enzyme is required in the leucine/isovalerate utilization, Liu, pathway for growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate, Liu proteins in metabolism of methyl-branched compounds, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
gene liuA as part of the liuABCDE gene cluster | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant LiuA from Escherichia coli | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyryl-CoA + 2,6-dichlorophenolindophenol | high substrate specificity | Pseudomonas aeruginosa | ? | - |
? | |
| isovaleryl-CoA + 2,6-dichlorophenolindophenol | high substrate specificity | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | the enzyme is required in the leucine/isovalerate utilization, Liu, pathway for growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate, Liu proteins in metabolism of methyl-branched compounds, overview | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | no activity of LiuA with isobutyryl-CoA, 3-hydroxybutyryl-CoA, octanoyl-CoA, citronellyl-CoA or 5-methyl-hex-4-enoyl-CoA | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 41000, recombinant LiuA, SDS-PAGE, 4 * 44000, LiuA, sequence calculation | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-CoA dehydrogenase | - |
Pseudomonas aeruginosa |
| LiuA | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | - |
Pseudomonas aeruginosa | |
| FAD | - |
Pseudomonas aeruginosa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
