KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
isovaleryl-CoA | sonic supernatant | Rattus norvegicus | |
0.0113 | - |
isovaleryl-CoA | - |
Cavia porcellus | |
0.0113 | - |
isovaleryl-CoA | purified enzyme in the absence of electron transfer agents | Rattus norvegicus | |
0.013 | - |
isovaleryl-CoA | whole sonicate | Rattus norvegicus | |
0.0132 | - |
isovaleryl-CoA | - |
Cavia porcellus | |
0.0132 | - |
isovaleryl-CoA | purified enzyme with electron-transferring flavoprotein and dichlorophenol indophenol, reaction terminated with KMnO4/HCl | Rattus norvegicus | |
0.0208 | - |
isovaleryl-CoA | - |
Cavia porcellus | |
0.0208 | - |
isovaleryl-CoA | purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with KMnO4/HCl | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | Cavia porcellus | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | Rattus norvegicus | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00003 | - |
intact mitochondria, no added acceptor | Rattus norvegicus |
0.00016 | - |
sonicated mitochondria, no added acceptor | Rattus norvegicus |
0.00044 | - |
sonicated mitochondria, acceptor added: electron-transferring flavoprotein | Rattus norvegicus |
0.00046 | - |
sonicated mitochondria, acceptor added: phenazine methosulfate | Rattus norvegicus |
0.0062 | - |
purified enzyme in the absence of electron transfer agents, reaction terminated with HCl or with KMnO4/HCl | Cavia porcellus |
0.0136 | - |
purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with HCl | Cavia porcellus |
0.0207 | - |
purified enzyme with electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with HCl, concentration of isovaleryl-CoA: 0.05 mM, specific activity determined by tritium release assay | Cavia porcellus |
0.0337 | - |
purified enzyme, acceptor added: electron-transferring flavoprotein | Cavia porcellus |
0.043 | - |
purified enzyme, acceptor added: electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with KMnO4/HCl, concentration of isovaleryl-CoA: 0.02 mM, specific activity determined by tritium release assay | Cavia porcellus |
0.043 | - |
purified enzyme, acceptor added: phenazine methosulfate | Cavia porcellus |
0.0472 | - |
purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with KMnO4/HCl | Cavia porcellus |
0.0572 | - |
purified enzyme with electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with KMnO4/HCl, concentration of isovaleryl-CoA: 0.05 mM, specific activity determined by tritium release assay | Cavia porcellus |
0.172 | - |
purified enzyme, acceptor added: electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, concentration of isovaleryl-CoA: 0.02 mM, specific activity determined by dye reduction assay | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | - |
Cavia porcellus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | - |
Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | enzyme activity observed in the absence of electron transfer agents suggests that rapid tritium exchange occurs between the enzyme-bound substrate and the incubation medium | Cavia porcellus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Cavia porcellus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? |