Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G185S | 80% of wild-type kcat with butanoyl-CoA | Homo sapiens |
R147W | 135% of wild-type kcat with butanoyl-CoA | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
butanoyl-CoA | 32°C, pH 7.0 | Homo sapiens | |
0.00045 | - |
butanoyl-CoA | 32°C, pH 7.0, R147W mutant | Homo sapiens | |
0.00075 | - |
butanoyl-CoA | 32°C, pH 7.0, G185S mutant | Homo sapiens | |
0.004 | - |
hexanoyl-CoA | 32°C, pH 7.0 | Homo sapiens | |
0.019 | - |
2-methylbutanoyl-CoA | 32°C, pH 7.0 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant SCAD | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.9 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbutanoyl-CoA + acceptor | - |
Homo sapiens | 2-methyl-2-butenoyl-CoA + reduced acceptor | - |
r | |
butanoyl-CoA + acceptor | - |
Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
r | |
hexanoyl-CoA + acceptor | - |
Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SCAD | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
15% loss of activity after 3 h, wild-type SCAD coexpressed with GroEL | Homo sapiens |
45 | - |
approx. 50% loss of activity after 3 h, R147W mutant expressed without GroEL | Homo sapiens |
45 | - |
approx. 50% loss of activity after 3 h, wild-type expressed without GroEL | Homo sapiens |
45 | - |
approx. 85% loss of activity after 3 h, G185S mutant expressed without GroEL | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
2-methylbutanoyl-CoA | 32°C, pH 7.0 | Homo sapiens | |
9.6 | - |
butanoyl-CoA | 32°C, pH 7.0 | Homo sapiens | |
10 | - |
hexanoyl-CoA | 32°C, pH 7.0 | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | no loss of activity after 5 h below 37°C in the presence of less than 3% glycerol | Homo sapiens |