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Literature summary for 1.3.8.1 extracted from

  • Nguyen, T.V.; Riggs, C.; Babovic-Vuksanovic, D.; Kim, Y.S.; Carpenter, J.F.; Burghardt, T.P.; Gregersen, N.; Vockley, J.
    Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme (2002), Biochemistry, 41, 11126-11133.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
G185S 80% of wild-type kcat with butanoyl-CoA Homo sapiens
R147W 135% of wild-type kcat with butanoyl-CoA Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
butanoyl-CoA 32°C, pH 7.0 Homo sapiens
0.00045
-
butanoyl-CoA 32°C, pH 7.0, R147W mutant Homo sapiens
0.00075
-
butanoyl-CoA 32°C, pH 7.0, G185S mutant Homo sapiens
0.004
-
hexanoyl-CoA 32°C, pH 7.0 Homo sapiens
0.019
-
2-methylbutanoyl-CoA 32°C, pH 7.0 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SCAD Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbutanoyl-CoA + acceptor
-
Homo sapiens 2-methyl-2-butenoyl-CoA + reduced acceptor
-
r
butanoyl-CoA + acceptor
-
Homo sapiens 2-butenoyl-CoA + reduced acceptor
-
r
hexanoyl-CoA + acceptor
-
Homo sapiens 2-hexenoyl-CoA + reduced acceptor
-
r

Synonyms

Synonyms Comment Organism
SCAD
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
15% loss of activity after 3 h, wild-type SCAD coexpressed with GroEL Homo sapiens
45
-
approx. 50% loss of activity after 3 h, R147W mutant expressed without GroEL Homo sapiens
45
-
approx. 50% loss of activity after 3 h, wild-type expressed without GroEL Homo sapiens
45
-
approx. 85% loss of activity after 3 h, G185S mutant expressed without GroEL Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
2-methylbutanoyl-CoA 32°C, pH 7.0 Homo sapiens
9.6
-
butanoyl-CoA 32°C, pH 7.0 Homo sapiens
10
-
hexanoyl-CoA 32°C, pH 7.0 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 no loss of activity after 5 h below 37°C in the presence of less than 3% glycerol Homo sapiens