Literature summary for 1.3.7.7 extracted from

Sarma, R.; Barney, B.M.; Hamilton, T.L.; Jones, A.; Seefeldt, L.C.; Peters, J.W.
Crystal structure of the L protein of Rhodobacter sphaeroides light-independent protochlorophyllide reductase with MgADP bound: a homologue of the nitrogenase Fe protein (2008), Biochemistry, 47, 13004-13015.

Search for more literature for 1.3.7.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Azotobacter vinelandii strain DJ884	Cereibacter sphaeroides

Crystallization (Commentary)

Crystallization (Comment)	Organism
L-protein of DPOR with Mg-ADP bound, microcapillary batch diffusion method, with 20-25% (w/v) PEG 3350 as the precipitating agent, with 200 mM magnesium formate (pH 7.7)	Cereibacter sphaeroides

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the purified, recombinant protein L contains 3.6 mol of Fe/mol of protein L homodimer, consistent with the presence of a [4Fe-4S] cluster	Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33310	-	calculated from amino acid sequence	Cereibacter sphaeroides
33380	-	mass spectroscopy	Cereibacter sphaeroides

Organism

Organism	UniProt	Comment	Textmining
Cereibacter sphaeroides	Q9RFD6	-	-

Purification (Commentary)

Purification (Comment)	Organism
zinc-loaded chelating Sepharose column chromatography and Q-Sepharose column chromatography	Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protochlorophyllide + oxidized ferredoxin + ADP + phosphate	-	Cereibacter sphaeroides	chlorophyllide a + reduced ferredoxin + ATP	-	?

Synonyms

Synonyms	Comment	Organism
dark-operative protochlorophyllide reductase	-	Cereibacter sphaeroides
DPOR	the DPOR holoenzyme is comprised of two component proteins, the dimeric protein L and the heterotetrameric NB-protein	Cereibacter sphaeroides
light-independent (dark) Pchlide reductase	-	Cereibacter sphaeroides
light-independent protochlorophyllide reductase	-	Cereibacter sphaeroides

Cofactor

Cofactor	Comment	Organism	Structure
4Fe-4S-center	-	Cereibacter sphaeroides
ADP	-	Cereibacter sphaeroides

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Release 2023.1 (January 2023)