Cloned (Comment) | Organism |
---|---|
recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin Ixalpha + reduced ferredoxin | Synechococcus sp. | - |
(3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
biliverdin Ixalpha + reduced ferredoxin | Synechococcus sp. 7002 | - |
(3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
- |
- |
Synechococcus sp. 7002 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin Ixalpha + reduced ferredoxin | - |
Synechococcus sp. | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
biliverdin Ixalpha + reduced ferredoxin | - |
Synechococcus sp. 7002 | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3Z-phycocyanobilin:ferredoxin oxidoreductase | - |
Synechococcus sp. |
PcyA | - |
Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Synechococcus sp. |
General Information | Comment | Organism |
---|---|---|
additional information | cyanobacteria utilize phycocyanobilin:ferredoxin oxidoreductase (PcyA) to perform a two-step reaction, the enzyme first reduces the 18-vinyl side chain of the D-ring and subsequently reduces the vinyl side chain of the pyrrole A ring to yield phycocyanobilin | Synechococcus sp. |