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Literature summary for 1.3.5.5 extracted from

  • Steinbrenner, J.; Sandmann, G.
    Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis (2006), Appl. Environ. Microbiol., 72, 7477-7484.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.5.5

Application

Application Comment Organism
synthesis the norflurazon-resistant phytoene desaturase gene is used for successful nuclear transformation and genetic engineering of the carotenoid biosynthesis pathway for accelerated astaxanthin biosynthesis (astaxanthin as a food supplement for humans) Haematococcus lacustris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Haematococcus lacustris

Protein Variants

Protein Variants Comment Organism
L504R the modified phytoene desaturase is still active in conversion of phytoene to zeta-carotene and exhibits 43fold-higher resistance to the bleaching herbicide norflurazon. The norflurazon-resistant phytoene desaturase gene is used for successful nuclear transformation and genetic engineering of the carotenoid biosynthesis pathway for accelerated astaxanthin biosynthesis Haematococcus lacustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + acceptor Haematococcus lacustris phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway 9,15,9'-tricis-zeta-carotene + reduced acceptor
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Organism

Organism UniProt Comment Textmining
Haematococcus lacustris
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 Flotow NIES-144
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + acceptor
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 Haematococcus lacustris 9,15,9'-tricis-zeta-carotene + reduced acceptor
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 ?
15-cis-phytoene + acceptor phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway Haematococcus lacustris 9,15,9'-tricis-zeta-carotene + reduced acceptor
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 ?