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Literature summary for 1.3.5.1 extracted from

  • Maklashina, E.; Hellwig, P.; Rothery, R.A.; Kotlyar, V.; Sher, Y.; Weiner, J.H.; Cecchini, G.
    Differences in protonation of ubiquinone and menaquinone in fumarate reductase from Escherichia coli (2006), J. Biol. Chem., 281, 26655-26664.
    View publication on PubMed
Search for more literature for 1.3.5.1

Protein Variants

Protein Variants Comment Organism
E29F mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione, the succinate-ubiquinone reductase reaction remains unaffected. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
E29F mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
E29L mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione, the succinate-ubiquinone reductase reaction remains unaffected. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
E29L mutation in subunit FrdC, dramatic decrease in enzymatic reactions with menaqunione. Elimination of the negative charge in E29 mutant enzymes results in significantly increased stabilization of both ubiquinone and menaquinone semiquinones Escherichia coli
K228L mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both menaquinone and ubiquinone Escherichia coli
K228L mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both ubiquinone and menaquinone Escherichia coli
K228R mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both menaquinone and ubiquinone Escherichia coli
K228R mutation in subunit FrdB. Residue K228 provides a strong hydrogen bond to menaquinone and is essential for reactions with both ubiquinone and menaquinone Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-n-heptyl-4-hydroxyquinoline-N-oxide
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 menaquinone mutant E29L, pH 7.0, 30°C Escherichia coli
0.004
-
 menaquinone wild-type, pH 7.0, 30°C Escherichia coli
0.01
-
 ubiquinone mutant E29F, pH 7.9, 30°C Escherichia coli
0.027
-
 ubiquinone mutant E29L, pH 7.9, 30°C Escherichia coli
0.07
-
 ubiquinone wild-type, pH 7.9, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + menaquinol
-
 Escherichia coli succinate + menaquinone
-
 r
additional information enzyme operates with both natural quinones, ubiquinone and menaquinone, at a single quinone binding site. Residue Lys228 in subunit FrdB provides a strong hydrogen bond to menaquinone and is essential for reactions with both quinone types. There is similar hydrogen bonding of the C1 carbonyl of both MQ and UQ, whereas there is different hydrogen bonding for their C4 carbonyls Escherichia coli ?
-
 ?
succinate + menaquinone
-
 Escherichia coli fumarate + menaquinol
-
 r
succinate + ubiquinone
-
 Escherichia coli fumarate + ubiquinol
-
 r

Synonyms

Synonyms Comment Organism
quinol-fumarate reductase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 succinate mutant K228R, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
0.3
-
 succinate mutant E49L, cosubstrate menaquinone, pH 7.9, 30°C Escherichia coli
0.3
-
 succinate mutant K228L, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
0.3
-
 fumarate mutant K228R, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli
0.4
-
 succinate mutant E49F, cosubstrate menaquinone, pH 7.9, 30°C Escherichia coli
2.3
-
 fumarate mutant E49F, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli
5
-
 fumarate mutant E49L, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli
15
-
 succinate wild-type, cosubstrate menaquinone, pH 7.9, 30°C Escherichia coli
20.4
-
 succinate mutant E49F, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
23
-
 succinate mutant E49L, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
24
-
 succinate wild-type, cosubstrate ubiquinone, pH 7.9, 30°C Escherichia coli
230
-
 fumarate wild-type, cosubstrate menaquinone, pH 7.0, 30°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00003
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide mutant E29L, pH 7.9, 30°C Escherichia coli
0.00005
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide wild-type, pH 7.0, 30°C Escherichia coli
0.00006
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide mutant E29L, pH 7.9, 30°C Escherichia coli
0.0002
-
 2-n-heptyl-4-hydroxyquinoline-N-oxide wild-type, pH 7.0, 30°C Escherichia coli