Protein Variants | Comment | Organism |
---|---|---|
H71Q | mutation in SdhC subunit, 82% and 69% of wild-type kcat with succinate-phenazine ethosulfate and succinate, respectively | Escherichia coli |
H84L | mutation in SdhC subunit, 54% and 23% of wild-type kcat with succinate-phenazine ethosulfate and succinate, respectively | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pentachlorophenol | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
succinate | 30°C, pH 7.8 | Escherichia coli | |
0.04 | - |
succinate | 30°C, pH 7.8, H84L mutant | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | [2Fe-2S], [4Fe-4S] and [3Fe-4S] clusters in SdhB subunit | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + ubiquinone | Escherichia coli | - |
fumarate + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant SQR | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + oxidized phenazine ethosulfate | - |
Escherichia coli | fumarate + reduced phenazine ethosulfate | - |
r | |
succinate + phenazine ethosulfate | - |
Escherichia coli | fumarate + reduced phenazine ethosulfate | - |
? | |
succinate + ubiquinone | - |
Escherichia coli | fumarate + ubiquinol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
complex II | - |
Escherichia coli |
SQR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
78 | - |
succinate | 30°C, pH 7.8 | Escherichia coli | |
82 | - |
oxidized phenazine ethosulfate | 30°C, pH 7.8 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein subunit SdhA | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
Pentachlorophenol | 30°C, pH 7.8 | Escherichia coli | |
0.083 | - |
Pentachlorophenol | 30°C, pH 7.8, H84L mutant | Escherichia coli |