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Literature summary for 1.3.5.1 extracted from

  • Matsson, M.; Ackrell, B.A.C.; Cochran, B.; Hederstedt, L.
    Carboxin resistance in Paracoccus denitrificans conferred by a mutation in the membrane-anchor domain of succinate:quinone reductase (complex II) (1998), Arch. Microbiol., 170, 27-37.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the succinate dehydrogenase CDAB operon from KTA4 mutant on plasmid in Escherichia coli Paracoccus denitrificans

Protein Variants

Protein Variants Comment Organism
B89G succinate dehydrogenase D polypeptide, the mutation conferrs resistance to carboxin but not to thenoyltrifluoroacetone Paracoccus denitrificans

Inhibitors

Inhibitors Comment Organism Structure
2-Thenoyltrifluoroacetone
-
Escherichia coli
2-Thenoyltrifluoroacetone inhibits both wild-type and carboxin-resistant enzymes, competitive inhibition with respect to quinone Paracoccus denitrificans
3'-bromo-carboxin not inhibitory Bacillus subtilis
3'-bromo-carboxin not inhibitory Escherichia coli
3'-bromo-carboxin inhibits Paracoccus denitrificans
3'-fluoro-carboxin not inhibitory Bacillus subtilis
3'-fluoro-carboxin not inhibitory Escherichia coli
3'-fluoro-carboxin inhibits Paracoccus denitrificans
3'-n-butyl-carboxin not inhibitory Bacillus subtilis
3'-n-butyl-carboxin not inhibitory Escherichia coli
3'-n-butyl-carboxin inhibits Paracoccus denitrificans
carboxin not inhibitory Bacillus subtilis
carboxin not inhibitory Escherichia coli
carboxin more than 90% inhibition in isolated membranes at high concentrations, the mutant enzyme is less sensitive than wild-type enzyme Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
ubiquinone mutant enzyme Paracoccus denitrificans
0.017
-
ubiquinone wild-type enzyme Paracoccus denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Paracoccus denitrificans 16020
-
membrane
-
Bacillus subtilis 16020
-
membrane
-
Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13900
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 Paracoccus denitrificans
14300
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 Paracoccus denitrificans
29600
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 Paracoccus denitrificans
65600
-
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Paracoccus denitrificans membrane-bound enzyme of the citric acid cycle and the respiratory chain ?
-
?
succinate + ubiquinone Paracoccus denitrificans
-
fumarate + ubiquinol
-
?
succinate + ubiquinone Bacillus subtilis
-
fumarate + ubiquinol
-
?
succinate + ubiquinone Escherichia coli
-
fumarate + ubiquinol
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
succinate dehydrogenase
-
Escherichia coli
-
succinate dehydrogenase
-
Paracoccus denitrificans
-
GenBank, Accession No. U31902
-

Purification (Commentary)

Purification (Comment) Organism
using solubilization with Thesit, column chromatography on HiLoad 26/10 Q-Sepharose and gel filtration on a Sephacryl S-300 column Paracoccus denitrificans

Storage Stability

Storage Stability Organism
-20°C, in 10 mM MOPS-HCl, pH 7.4, 2-4% Thesit, 50% glycerol, stable for at least a few weeks Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + naphthoquinol
-
Paracoccus denitrificans succinate + naphthoquinone
-
r
additional information membrane-bound enzyme of the citric acid cycle and the respiratory chain Paracoccus denitrificans ?
-
?
succinate + ubiquinone
-
Paracoccus denitrificans fumarate + ubiquinol
-
?
succinate + ubiquinone
-
Bacillus subtilis fumarate + ubiquinol
-
?
succinate + ubiquinone
-
Bacillus subtilis fumarate + ubiquinol
-
r
succinate + ubiquinone
-
Escherichia coli fumarate + ubiquinol
-
?
succinate + ubiquinone
-
Escherichia coli fumarate + ubiquinol
-
r
succinate + ubiquinone determination of quinol:fumarate reductase activity using 2,3-dimethyl-1,4-naphthoquinol Paracoccus denitrificans fumarate + ubiquinol
-
r

Subunits

Subunits Comment Organism
tetramer
-
Escherichia coli
tetramer 1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 Paracoccus denitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
naphthoquinol mutant enzyme Paracoccus denitrificans
2
-
naphthoquinol wild-type enzyme Paracoccus denitrificans
10
-
succinate mutant enzyme Paracoccus denitrificans
260
-
succinate wild-type enzyme Paracoccus denitrificans