Cloned (Comment) | Organism |
---|---|
cloning of the succinate dehydrogenase CDAB operon from KTA4 mutant on plasmid in Escherichia coli | Paracoccus denitrificans |
Protein Variants | Comment | Organism |
---|---|---|
B89G | succinate dehydrogenase D polypeptide, the mutation conferrs resistance to carboxin but not to thenoyltrifluoroacetone | Paracoccus denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Thenoyltrifluoroacetone | - |
Escherichia coli | |
2-Thenoyltrifluoroacetone | inhibits both wild-type and carboxin-resistant enzymes, competitive inhibition with respect to quinone | Paracoccus denitrificans | |
3'-bromo-carboxin | not inhibitory | Bacillus subtilis | |
3'-bromo-carboxin | not inhibitory | Escherichia coli | |
3'-bromo-carboxin | inhibits | Paracoccus denitrificans | |
3'-fluoro-carboxin | not inhibitory | Bacillus subtilis | |
3'-fluoro-carboxin | not inhibitory | Escherichia coli | |
3'-fluoro-carboxin | inhibits | Paracoccus denitrificans | |
3'-n-butyl-carboxin | not inhibitory | Bacillus subtilis | |
3'-n-butyl-carboxin | not inhibitory | Escherichia coli | |
3'-n-butyl-carboxin | inhibits | Paracoccus denitrificans | |
carboxin | not inhibitory | Bacillus subtilis | |
carboxin | not inhibitory | Escherichia coli | |
carboxin | more than 90% inhibition in isolated membranes at high concentrations, the mutant enzyme is less sensitive than wild-type enzyme | Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
ubiquinone | mutant enzyme | Paracoccus denitrificans | |
0.017 | - |
ubiquinone | wild-type enzyme | Paracoccus denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Paracoccus denitrificans | 16020 | - |
membrane | - |
Bacillus subtilis | 16020 | - |
membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13900 | - |
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 | Paracoccus denitrificans |
14300 | - |
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 | Paracoccus denitrificans |
29600 | - |
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 | Paracoccus denitrificans |
65600 | - |
1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Paracoccus denitrificans | membrane-bound enzyme of the citric acid cycle and the respiratory chain | ? | - |
? | |
succinate + ubiquinone | Paracoccus denitrificans | - |
fumarate + ubiquinol | - |
? | |
succinate + ubiquinone | Bacillus subtilis | - |
fumarate + ubiquinol | - |
? | |
succinate + ubiquinone | Escherichia coli | - |
fumarate + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
succinate dehydrogenase | - |
Escherichia coli | - |
succinate dehydrogenase | - |
Paracoccus denitrificans | - |
GenBank, Accession No. U31902 | - |
Purification (Comment) | Organism |
---|---|
using solubilization with Thesit, column chromatography on HiLoad 26/10 Q-Sepharose and gel filtration on a Sephacryl S-300 column | Paracoccus denitrificans |
Storage Stability | Organism |
---|---|
-20°C, in 10 mM MOPS-HCl, pH 7.4, 2-4% Thesit, 50% glycerol, stable for at least a few weeks | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + naphthoquinol | - |
Paracoccus denitrificans | succinate + naphthoquinone | - |
r | |
additional information | membrane-bound enzyme of the citric acid cycle and the respiratory chain | Paracoccus denitrificans | ? | - |
? | |
succinate + ubiquinone | - |
Paracoccus denitrificans | fumarate + ubiquinol | - |
? | |
succinate + ubiquinone | - |
Bacillus subtilis | fumarate + ubiquinol | - |
? | |
succinate + ubiquinone | - |
Bacillus subtilis | fumarate + ubiquinol | - |
r | |
succinate + ubiquinone | - |
Escherichia coli | fumarate + ubiquinol | - |
? | |
succinate + ubiquinone | - |
Escherichia coli | fumarate + ubiquinol | - |
r | |
succinate + ubiquinone | determination of quinol:fumarate reductase activity using 2,3-dimethyl-1,4-naphthoquinol | Paracoccus denitrificans | fumarate + ubiquinol | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Escherichia coli |
tetramer | 1 * 65600 + 1 * 29600 + 1 * 14300 + 1 * 13900 | Paracoccus denitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
naphthoquinol | mutant enzyme | Paracoccus denitrificans | |
2 | - |
naphthoquinol | wild-type enzyme | Paracoccus denitrificans | |
10 | - |
succinate | mutant enzyme | Paracoccus denitrificans | |
260 | - |
succinate | wild-type enzyme | Paracoccus denitrificans |