Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Thenoyltrifluoroacetone | - |
Bos taurus | |
carboxin | Q2-mediated Wurster's blue reduction: 90% inhibition at 0.02 mM, Q0-mediated Wurster's blue reduction: 75% inhibition at 0.02 mM, Q1-mediated Wurster's blue reduction: 88% inhibition at 0.02 mM, Q6-mediated Wurster's blue reduction: 50% inhibition at 0.02 mM | Bos taurus | |
fumarate | - |
Bos taurus | |
malonate | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
ubiquinone-2 | - |
Bos taurus | |
0.02 | - |
succinate | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
succinate dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
using urea washing, Triton X-100 extraction, cholate-ammonium sulfate extraction, calcium phosphate gel and ammonium sulfate precipitation | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | 54 | depending on assay temperature | Bos taurus |
Storage Stability | Organism |
---|---|
liquid N2 or -20°C, pH 6.0, presence of 0.1% Triton X-100 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone | - |
Bos taurus | fumarate + 2,3-dimethoxy-5-methyl-6-geranyl-1,4-benzoquinol | - |
? | |
succinate + ubiquinone | - |
Bos taurus | fumarate + ubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | heart, succinate dehydrogenase, SDS-PAGE | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.3 | 150 | succinate | based on FAD content, dependency on assay temperature | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | inactivation below pH 5, stable for about 20 h without loss of activity at pH 6.0 in the presence of 0.1% Triton X-100 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b | - |
Bos taurus | |
cytochrome b-560 | - |
Bos taurus | |
FAD | - |
Bos taurus | |
additional information | the enzyme contains acid-labile sulfides | Bos taurus | |
ubiquinone | - |
Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
malonate | - |
Bos taurus | |
0.001 | - |
carboxin | - |
Bos taurus | |
0.008 | - |
2-Thenoyltrifluoroacetone | - |
Bos taurus | |
0.04 | - |
fumarate | - |
Bos taurus |