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Literature summary for 1.3.5.1 extracted from

  • Unden, G.; Kr๖ger, A.
    The function of the subunits of the fumarate reductase complex of Vibrio succinogenes (1981), Eur. J. Biochem., 120, 577-584.
    View publication on PubMed
Search for more literature for 1.3.5.1

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes
31000
-
 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes
79000
-
 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fumarate + 2,3-dimethyl-1,4-naphthohydroquinone Wolinella succinogenes
-
 succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 ?
additional information Wolinella succinogenes the fumarate reductase complex has two different reactive sites, which are essential for its function in the phosphorylative electron transport of the bacterium ?
-
 ?

Organism

Organism UniProt Comment Textmining
Wolinella succinogenes
-
 formerly Vibrio succinogenes, fumarate reductase
-

Renatured (Commentary)

Renatured (Comment) Organism
the cleavage of the complex causes the complete loss of activity of fumarate reduction by dimethylnaphthohydroquinone, the activity can be restored by coprecipitation of the three subunits of MW: 79000 Da, 31000 Da and 25000 Da Wolinella succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fumarate + 2,3-dimethyl-1,4-naphthohydroquinone
-
 Wolinella succinogenes succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 ?
fumarate + 2,3-dimethyl-1,4-naphthohydroquinone reaction catalyzed by fumarate reductase complex, the site of the complex reacting with fumarate is situated on the 79000 Da subunit, and the site reacting with dimethylnaphthohydroquinone is cytochrome b Wolinella succinogenes succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 ?
fumarate + 2,3-dimethyl-1,4-naphthohydroquinone reaction catalyzed by fumarate reductase complex, the site of the complex reacting with fumarate is situated on the 79000 Da subunit, and the site reacting with dimethylnaphthohydroquinone is cytochrome b Wolinella succinogenes succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 r
additional information the fumarate reductase complex has two different reactive sites, which are essential for its function in the phosphorylative electron transport of the bacterium Wolinella succinogenes ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 fumarate based on the content of the MW 79000 peptide, reduction of fumarate with dimethylnaphthohydroquinone Wolinella succinogenes

Cofactor

Cofactor Comment Organism Structure
cytochrome b has a MW of 25000 Da, a midpoint potential of - 15 mV and is reducible by dimethylnaphthohydroquinone in the absence of the other subunits Wolinella succinogenes
additional information measurement of the redox potentials of the sulfur-centers Wolinella succinogenes