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Literature summary for 1.3.3.8 extracted from
- Molecular characterization, recombinant expression in Escherichia coli and biological activity of (S)-Tetrahydroberberine oxidase from Corydalis saxicola Bunt (2012), Mol. Biol. Rep., 39, 3319-3326.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Cs(S)-THBO, DNA and amino acid sequence determination and analysis, real-time quantitative PCR analysis, tissue expression profiling analyses, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Corydalis saxicola |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 43000, recombinant His-tagged enzyme, SDS-PAGE, x * 25000, recombinant detagged enzyme | Corydalis saxicola |
| 43000 | - |
x * 43000, recombinant His-tagged enzyme, SDS-PAGE, x * 25000, recombinant detagged enzyme | Corydalis saxicola |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-tetrahydroberberine + 2 O2 | Corydalis saxicola | - |
berberine + 2 H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corydalis saxicola | G0XRK4 | gene Cs(S)-THBO | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Corydalis saxicola | - |
| leaf | - |
Corydalis saxicola | - |
| additional information | the enzyme is constitutively expressed in roots, stems, leaves and flowers, with the highest expression level in roots, real-time quantitative PCR analysis, tissue expression profiling analyses, overview | Corydalis saxicola | - |
| root | - |
Corydalis saxicola | - |
| stem | - |
Corydalis saxicola | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-tetrahydroberberine + 2 O2 | - |
Corydalis saxicola | berberine + 2 H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, recombinant His-tagged enzyme, SDS-PAGE, x * 25000, recombinant detagged enzyme | Corydalis saxicola |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (S)-tetrahydroberberine oxidase | - |
Corydalis saxicola |
| (S)-THB oxidase | - |
Corydalis saxicola |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | (S)-tetrahydroberberine oxidase is the last enzyme of benzylisoquinoline alkaloids pathway which catalyzes the dehydrogenation of four hydrogen atoms of (S)-THB to produce berberine, the final step of berberine biosynthesis | Corydalis saxicola |
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